基于邻苯二胺聚合膜的纳米金自组装NADH修饰电极测定肾上腺素  

A Novel Sensor of NADH Immobilized on Poly-o-phenylenediamine Film Self-Assembled Nano Gold and Its Application for the Determination of Epinephrine

作　　者：黄艳子[1] 罗红群[1] 李念兵[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2007年第11期70-74,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20575054);重庆市自然科学基金资助项目(CSTC-2006BB0342)

摘　　要：研究了基于邻苯二胺聚合膜的纳米金自组装NADH修饰玻碳电极的制备，并探讨了肾上腺素在此修饰电极上的电化学行为，在pH=7．2的磷酸盐缓冲液中，肾上腺素在该修饰电极上产生一个灵敏的氧化峰，峰电流与肾上腺素浓度在3．0×10^-6～5．0×10^-5mol／L范围内呈良好的线性关系，检出限为3．15×10^-7mol／L．该方法可用于盐酸肾上腺素注射液中肾上腺素的检测．The preparation of NADH-poly-o-phenylenediamine film-nano gold chemically modified glassy carbon electrode was studied, and the cyclic voltametric behavior of epinephrine （EP） on this modified electrode was investigated. EP showed a sensitive oxidation peak on the modified electrode in a phosphate buffer solution （PBS） of pH=7.2. The linear range of EP was 3. 0×10^-6-5. 0×10^-5 mol/L and the detection limit was 5.0 ×10^-7 mol/L. This method was applied to the measurement of EP in epinephrine hydrochloride injection samples with satisfactory results.

关 键 词：烟酰胺腺嘌呤二核苷酸(辅酶I NADH) 纳米金 邻苯二胺电聚合膜 肾上腺素 

分 类 号：O657.15[理学—分析化学]