高速逆流色谱结合大孔树脂从龙胆中快速分离高纯度龙胆苦苷  

Preparative separation of Gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin

作　　者：许有威[1] 齐艳[1] 韩旭[1] 许丽娜[1] 徐奇玮[1] 彭金咏[1] 

机构地区：[1]大连医科大学药学院,大连116027

出　　处：《中国中药杂志》2007年第24期2595-2597,共3页China Journal of Chinese Materia Medica

摘　　要：目的:建立龙胆中高纯度龙胆苦苷的快速分离制备方法。方法:药材醇提取液经D101大孔吸附树脂柱的层析物直接进行高速逆流色谱(high-speed counter-current chromatography,HSCCC)分离纯化,以醋酸乙酯-正丁醇-水(2∶1∶3)为溶剂系统,下相为流动相,流速1.5 mL.min-1,检测波长254 nm,分离温度30℃,所得产物经高效液相色谱与质谱分析检测,并与标准品对照。结果:在此条件下,经一步分离从300 mg粗提物中得到136 mg纯度为99.6%的龙胆苦苷。结论:该法简便、快速、所得产物纯度高,适合于龙胆苦苷标准品的制备。Objective： To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography （HSCCC）. Method： The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography （HSCCC）. A two-phase solvent system composed of ethyl acetate： n-butanol： water （2： 1： 3） was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL · min^-1 , while the apparatus rotated at 800 r · min^-1 and the eluate was detected at 254 nm. Result： 136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes. Conclusion： The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.

关 键 词：龙胆 高速逆流色谱 大孔树脂 龙胆苦苷 

分 类 号：R284[医药卫生—中药学]