巴戟天多糖亲和柱层析纯化的研究  

Study on Affinity Chromatography Purification of Morinda officinalis How Polysaccharides

作　　者：江咏[1] 李琳[1] 陈玲[1] 张喜梅[1] 李晓玺[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品科学》2007年第12期95-99,共5页Food Science

基　　金：广东省科技攻关项目(2003C104025)

摘　　要：通过静态吸附的方法探讨了巴戟天粗多糖在Con A Sephorose4B亲和填料上静态吸附和脱附情况。结果表明,吸附过程中的pH值和离子强度对平衡吸附量有明显影响,得到的最佳吸附条件是pH7.5,NaCl浓度0.1mol/L;确定的最佳脱附条件为是pH6.5,α-D-甲基葡萄糖苷浓度0.02mol/L。由Langmuir方程对实验数据进行拟合,得到填料的最大吸附量qm为9.58mg/g,表观解离常数Kd为3.43mg/g,巴戟天粗多糖在填料上的吸附速率K为0.144mg/h。在最佳的吸附和洗脱条件下,巴戟天粗多糖被分为MOP-A1和MOP-A2两个组分。The isolation of water-soluble polysaccharides （MOP） from Morinda officinalis How was discussed in detail with affinity chromatography. The static adsorption experiments of MOP revealed that the optimum conditions for the adsorption are pH 7.0 and NaCl concentration 0.1 mol/L, and optimum conditions for desorption are： pH 6.0 and the concentration of α- D-methylglucoside 0.02 mol/L. After fitting the experiments data with Langmuir equation, several adsorption parameter for MOP were obtained as： adsorption capacity qm 9. 58 mg/g, dissociation constant Kd 3.43 mg/g, and adsorption rate K 0.144 mg/h. The dynamics absorption of MOP on Con A Sepharose 4B chromatography column were investigated by breakthrough curve. Two fractions, MOP-A1 and MOP-A2 were obtained under the optimum adsorption and elution conditions.

关 键 词：巴戟天 多糖 亲和层析 吸附 

分 类 号：R284.2[医药卫生—中药学]