H_2O_2对骨骼肌ryanodine受体的氧化作用  

The Oxidization From H_2O_2 to the Skletal Muscle Ryanodine Receptor

作　　者：王清爽[1] 夏冰[1] 于源华[1] 

机构地区：[1]长春理工大学生命科学技术学院,长春130022

出　　处：《长春理工大学学报（自然科学版）》2007年第4期66-68,65,共4页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省教育厅资助项目(2004JYT08)

摘　　要：Ryanodine受体(RyR)是肌质网快速释放Ca2+的通道,在细胞钙信号转导及相关生理过程如肌细胞的兴奋-收缩偶联过程中扮演重要角色。SDS-PAGE和免疫印迹实验结果显示H2O2能够氧化SR膜上的RyR1,使其560-kDa单体和410-kDa内源性酶解片段的强度降低直至消失,并产生三个高分子量复合物。加入还原剂二巯基乙醇,能够抵消这种氧化作用。Ryanodine receptor （ RyR ） is the fast Ca^2＋ release channel of the sarcoplasmic reticulum and plays a vital role in Ca^2＋ signaling and excitation-contraction coupling in muscle cells. Upon treatment ofsarcoplasmic reticulum with H2O2, SDS-PAGE showed that the intensity of the 565-kDa RyR1 peptided and its 410-kDa endogenous enzymatic fragment （fRyR） was dramatically reduced, and in parallel, three high-molecular-weight bands were observed. Western blotting analysis revealed that these high-molecular-weight bands were cross-linked RyR subunits. The chemical changes of RyR1 were reversible upon reduction by reducing agent 2-mercaptoethanol.

关 键 词：骨骼肌ryanodine受体 胰蛋白酶 过氧化氢 

分 类 号：Q51[生物学—生物化学]