地黄连作对根际微生物区系及土壤酶活性的影响  

Effects of successive cropping Rehmannia glutinosa on rhizosphere soil microbial flora and enzyme activities.

作　　者：陈慧[1] 郝慧荣[1] 熊君[1] 齐晓辉[1] 张重义[1] 林文雄[1] 

机构地区：[1]福建农林大学农业生态研究所,福州350002

出　　处：《应用生态学报》2007年第12期2755-2759,共5页Chinese Journal of Applied Ecology

基　　金：国家"十一五"科技支撑计划重点项目(2006BAI09B03);福建省科技重大项目(2004YZ02;2004YZ022;2004YZ024);国家自然科学基金资助项目(30772729).

摘　　要：以地黄连作2年和1年的土壤为研究对象,分别测定了根际微生物区系变化及根际土壤酶活性.结果表明:地黄连作对其根际微生物区系及土壤酶活性产生了较大的影响.随种植年限的增加,根际细菌和真菌减少,但差异均不显著;放线菌增多,连作2年的土壤约为1年的4倍.土壤中氨化细菌、好气性固氮菌、硫化细菌、反硝化细菌和嫌气性纤维素分解菌分别增加了25.99、45.39、11.43、1.36和1.43倍,而好气性纤维素分解菌减少了86.74%.连作地黄根系的分泌物对脲酶、多酚氧化酶、蔗糖酶、蛋白酶和纤维素酶活性具有促进作用,分别增加了62.87%、9.43%、47.91%、139.62%和31.33%,而对过氧化氢酶则呈抑制作用.说明地黄连作会破坏根际微生物种群平衡.With the soils cropped Rehmannia glutinosa for one and two years as test materials, this paper studied the variations of microbial flora and enzyme activities in rhizosphere. The results showed that with increasing year of cropping, the numbers of bacteria and fungi in rihzosphere had a slight decrease. In the soil successively cropped R. glutinosa for two years, the number of aetinomyeetes in rihzosphere was 4 times higher, the ammonifier, aerobic azotobaeter, sulphate reducer, denitrifier, and anaerobic cellulose-decomposer increased by 25. 99, 45. 39, 11.43, 1.36, and 1.43 folds, respectively, while aerobic cellulose-decomposer reduced by 86. 74% , compared with those in the soil cropped R. glutinosa for one year. The root exudates of successively cropped R. glutinosa increased the activities of urease, polyhphenol oxidase, sucrase, protease and cellulase in rhizosphere by 62. 87%, 9. 43%, 47.91%, 139. 62% and 31.33%, respectively, but inhibited the activity of catalase. The findings suggested that successive cropping R. glutinosa would destroy the balance of rhizosphere soil microbial flora.

关 键 词：地黄 连作障碍 根际微生物 土壤酶活性 

分 类 号：S567.234[农业科学—中草药栽培]