核桃楸叶乙醇提取物杀虫活性及活性成分  

Insecticidal activity and active components of alcohol extract from Juglans mandshurica Maxim leaves.

作　　者：孙墨珑[1] 宋湛谦 方桂珍[3] 

机构地区：[1]东北林业大学理学院,哈尔滨150040 [2]中国林业科学研究院林产化学工业研究所,南京210042 [3]东北林业大学材料科学与工程学院,哈尔滨150040

出　　处：《应用生态学报》2007年第12期2910-2914,共5页Chinese Journal of Applied Ecology

基　　金：哈尔滨市留学回国人员基金资助项目(2005AFLXJ005).

摘　　要：采用常规提取法浸提核桃楸叶的杀虫活性成分.结果表明,核桃楸叶乙醇提取物和乙醇提取物氯仿萃取相对舞毒蛾幼虫和甘蓝夜蛾幼虫具有杀虫活性.施药5 d,药液浓度≥10g.L-1时,核桃楸叶乙醇提取物和氯仿萃取相对舞毒蛾幼虫和甘蓝夜蛾幼虫的触杀作用和胃毒作用的校正死亡率均超过50%.核桃楸叶乙醇提取物对舞毒蛾幼虫和甘蓝夜蛾幼虫的触杀作用和胃毒作用强于氯仿萃取相.采用气-质联用技术GC-MS分析核桃楸叶乙醇提取物中氯仿萃取相的化学组成和相对含量.结果表明:核桃楸叶乙醇提取物中氯仿萃取相的主要杀虫活性成分为相对含量最高的胡桃醌,即5-羟基-1,4-萘醌;其他杀虫活性成分可能为对称2,2’-亚甲基6-(1,1-二甲乙基)4-甲基双酚和2-甲氧基4-乙烯基苯酚.The insecticidal active components in Juglans mandshurica Maxim leaves were extracted by conventional method. The alcohol extract and its chloroform-extraction phase showed insecticidal activities in contact toxicity and stomach toxicity against Lymantria dispar L. and Mamestra brassicae L. larvae. When the concentration of the extract was above 10 g · L^-1 , the corrected mortality was higher than 50% 5 days after applying the extract. Alcohol extract had higher insecticidal activities than its chloroform-extraction phase. The GC-MS analysis on the active components in the chloroform-extraction phase showed that the main component was juglone （5-hydroxy-1,4-naphthalenedione）, and the others were 2,2＇-methylenebis-6-（ 1,1-dimethylethyl）-d-methyl- diphenol and 2- methoxy-4-vinylphenol.

关 键 词：核桃楸 杀虫活性 成分分析 GC—MS 胡桃醌 

分 类 号：TQ450.21[化学工程—农药化工]