注射自体成纤维细胞除皱的临床应用  

Clinical application of autologous fibroblasts in treatment of facial wrinkles

作　　者：石杭燕[1] 孙燚[1] 严晟[1] 陈达[1] 郭金才[1] 应彬彬[1] 张志民[1] 吴溯帆[1] 

机构地区：[1]浙江省人民医院整形外科,杭州310014

出　　处：《中华医学美学美容杂志》2007年第6期321-322,共2页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的观察体外培养的自体成纤维细胞注射的面部除皱临床效果,探讨其在面部年轻化治疗中的意义。方法取10例睑袋及上睑松弛手术切除的皮肤行体外培养,细胞数量扩增至108后注射入面部不同治疗部位皮肤的真皮深层,共注射3次,每次间隔2周。结果10例随访6-12月,各例皱纹均得到明显改善,未出现免疫排斥、肉芽肿等并发症。结论应用培养的自体成纤维细胞注射去除面部浅表皱纹,治疗过程简便、创伤微小、安全、效果确实,是一种较好地填充除皱方法。由于尚缺乏更长期的随访结果,远期疗效还有待进一步观察。Objective To treat facial wrinkles with autologous fibroblast injection, to investigate its curative effects and significances, and to evaluate its clinical efficacy. Methods Ten patients were treated by this method. Their excessive skin removed from operations of low eyelid pouched or upper eyelid chalasis were sent to the laboratory for fibroblast culture in vitro at 37°C and 5% CO2. About 108 fibroblasts were injected into the deep layer of dermis after 4 to 6 week’s culture. When the wrinkles were corrected satisfactorily, additional fibroblasts were injected to achieve an overcorrection effect. The injections were performed 3 times. The interval between each injection was 2 weeks. Long-term follow-ups from 6 months to 1 year were performed. Results The wrinkles were successfully improved after treatment. The injected fibroblasts remained in situ without evidence of significant resorption or loss of correction. Most patients were satisfied with the achieved long-term results. No obvious complications such as immunologic rejection, granuloma, hematoma, hypersensitiveness, etc. were observed. Conclusion This method is an easy, safe and mini-invasive approach to treat facial wrinkles with good effect. It may be used as a living, dynamic repair system for dermal and subcutaneous deficiencies. But its long-term effects are still needed to further follow up.

关 键 词：成纤维细胞 自体移植 面部皱纹 

分 类 号：R6[医药卫生—外科学]