β1转化生长因子对瘢痕疙瘩成纤维细胞分泌相关的基质金属蛋白酶的影响  

Effect of TGF-β1 on the seretion of matrix metalloproteinase for migration in human keloid-derived fibroblasts

作　　者：缪泽群[1] 李航[2] 

机构地区：[1]武汉大学中南医院皮肤科,武汉430071 [2]北京大学第一医院皮肤科

出　　处：《中华医学美学美容杂志》2007年第6期355-357,共3页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的探讨β1转化生长因子（TGF-β1）对瘢痕疙瘩成纤维细胞分泌基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-2（MMP-2）和金属蛋白酶组织抑制剂-1（TIMP-1）表达的调节作用,及TGF-β1与瘢痕的关系。方法培养的瘢痕疙瘩成纤维细胞经TGF-β1处理,采用酶联免疫法（ELISA）测定瘢痕疙瘩成纤维细胞上清液中MMP-1、MMP-2和TIMP-1的水平。结果瘢痕疙瘩中成纤维细胞MMP-1、MMP-2和TIMP-1的生成量显著高于正常皮肤（P〈0.01）;加TGF-β1处理后,瘢痕疙瘩中成纤维细胞MMP-1的分泌量显著降低（P〈0.01）,MMP-2的分泌量显著升高（P〈0.01）,而TIMP-1的分泌量无显著改变（P〉0.05）。结论培养的瘢痕疙瘩成纤维细胞显示MMP-1、MMP-2和TIMP-1增加,TGF-β1在生成调节过程中起重要作用。Objective To investigate the regulatory effects of transforming growth factor-β1 on the secretion of matrix metalloproteinase-1 （MMP-1）, matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in human keloid-derived fibroblasts. Methods The cultured keloid fibroblasts were treated with TGF-β1. Enzyme-linked immunosorbent assay （ELISA） method were performed to measure the production of MMP-1, MMP-2 and TIMP-1 in human keloid fibroblasts supernatant. Results The production of MMP-1, MMP-2 and TIMP-1 in human keloid fibroblasts were significantly higher than those in normal dermal fibroblasts （P〈0.01）; after TGF-β1 was added to the cultures, the production of MMP-1 was decreased significantly （P〈0.01）, the production of MMP-2 was increased significantly （P〈0.01）, and production of TIMP-1 did not change significantly （P〉0.05）. Conclusions Cultured keloid fibroblasts show increased production of MMPs, and TGF-β1 plays a role in this regulation of production.

关 键 词：瘢痕疙瘩 成纤维细胞 Β1转化生长因子 基质金属蛋白酶-1 基质金属蛋白酶-2 金属蛋白酶组织抑制剂-1 

分 类 号：R6[医药卫生—外科学]