氯霉素残留伏安免疫测试法的电化学条件  

Electrochemical conditions for immunovoltammetry-detection of chloramphenicol residues

作　　者：宋巍巍[1] 丁明星[1] 刘国艳[2] 张挪威[1] 柴春彦[2] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]上海交通大学农业与生物学院,上海201101

出　　处：《中国兽医科学》2007年第12期1090-1093,共4页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30571368);上海市科委攻关计划项目(06dz05040)

摘　　要：在间接竞争ELISA的基础上，建立了以碱性磷酸酶（ALP）-对硝基苯磷酸（pNPP）为反应体系的检测氯霉素（CAP）残留的伏安免疫方法，并对此法的电化学条件进行了详细的探讨。结果，在0．2mol／L的B-R电解质溶液（pH3．0）中，当扫描速度为50mV／s时，伏安法对硝基苯酚（PNP）的灵敏度最佳；且在1．2V处，该物质具有良好的氧化峰形。用此法测试CAP的检测下限达到0．064ng／mL，检测线性范围为0．15～600ng／mL。To chloramphenicol r explore immunosensor technique with high sensitivity and specificity for determination of esidues in milk, an immunovoltammetric method was developed by using ALP-pNPP as reacting system based on ciELISA,and conditions for immunovoltammetric determination of chloramphenicol were discussed in detail. The results showed that high sensitivity of the voltammetric determination of PNP was manifested in the supporting solution 0. 2 mol/L Britton-Robinson buffer（pH3.0） with a scanning rate at 50 mV/s and finely-sharped oxidative peak current was shown when the potential was at 1.2 V. The chloramphenicol detection limit of the method was 0. 064 ng/mL within the linear range of 0.15- 600 ng/mL.

关 键 词：氯霉素 伏安免疫法 氧化峰电流 电化学条件 

分 类 号：S859.84[农业科学—临床兽医学]