超小超顺磁性氧化铁微粒对神经干细胞生物学活性的影响  

Influence of ultrasmail superparamagnefic iron oxide sinerem on biologic activity of neural stem cells

作　　者：樊娟[1] 徐如祥[1] 姜晓丹[1] 陈中灿[1] 裴少琨[1] 杨志军[1] 王娆[1] 邹雨汐[1] 

机构地区：[1]南方医科大学附属珠江医院神经医学研究所,广州510282

出　　处：《中华创伤杂志》2007年第12期885-888,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30400464）;广东省名医工程研究资助项目（粤卫[2004]199号）;中国科学院武汉物理数学研究所质谱与原子分子物理国家重点实验室基金资助项目（T152606）;广东省科技计划资助项目（20062070088）;广州市科技计划资助项目（2006B36004013）

摘　　要：目的应用超小超顺磁性氧化铁微粒Sinerem标记大鼠骨髓源性神经干细胞（NSCs），探讨Sinerem的安全性及合适的标记浓度。方法分离、培养大鼠骨髓源性NSCs。制备Sinerem多聚赖氨酸复合物，以不同浓度Sinerem对细胞进行标记。普鲁士蓝染色和电镜观察细胞内铁，CCK-8法检测细胞生长增殖情况，AnnexinV—PI法检测细胞凋亡及死亡情况。结果普鲁士蓝染色显示细胞质内大量铁颗粒存在，标记率在99％以上。电镜观察见Sinerem标记干细胞内含纳米铁颗粒。CCK-8法检测结果表明，25～1500μg/ml不同浓度范围的Sinerem对细胞增殖的影响差异无统计学意义。AnnexinV—PI检测结果显示，Sinerem在25～200μg/ml范围内的不同浓度对细胞凋亡和死亡的影响差异无统计学意义。结论超小超顺磁性氧化铁微粒Sinerem可以有效标记大鼠骨髓源性NSCs，可以应用25～200μg／ml浓度范围的Sinerem标记细胞。Objective To label the bone marrow-derived neural stem cells （NSCs） with Sinerem, the uhrasmall superparamagnetic iron oxide （USPIO） and determine the safety of Sinerem as well as its suitable concentration. Methods Bone marrow-derived NSCs and Sinerem-polylysine （ Sinerem- PLL） complex were prepared. The cells were labeled with the Sinerem-PLL complex of different concentrations. The nanoparticles in the cytoplasm were observed with Prussian blue staining and electron microscopyto. Cell growth viability, apoptosis and cell death were detected by CCK-8 and AnnexinV/PI tests respectively. Results Prussian blue staining showed numerous iron particles in the cytoplasm with a labeling rate of over 99%. Iron particles were observed in Sinerem-labeled stem cells with electron microscopy. The CCK-8 and AnnexinV/PI tests showed that Sinerem of different concentrations （25- 200 μg/ml） had no significantly different influence on cell growth viability, apoptosis and death. Conclusion Labeling bone marrow-derived NSCs with Sinerem （25-200 μg/ml） is safe and efficient.

关 键 词：干细胞 氧化铁 细胞生物学 标记 细胞 造影剂 

分 类 号：R686[医药卫生—骨科学]