高效液相色谱法测定联磺甲氧苄啶片中磺胺嘧啶含量  

Determination of Sulfadiazine in Sulfamethoxazole,Suladiazine and Trimethoprim Tablet by HPLC

作　　者：刘宏英 

机构地区：[1]黑龙江省鸡西市药品检验所,黑龙江鸡西158100

出　　处：《中国药业》2007年第24期29-29,共1页China Pharmaceuticals

摘　　要：目的建立测定联磺甲氧苄啶片中磺胺嘧啶的高效液相色谱(HPLC)法。方法采用ODSC18色谱柱(150mm×4.6mm,5μm),以水-乙腈-三乙胺(799∶200∶1,用氢氧化钠试液或冰醋酸调pH值至5.9)为流动相,检测波长为241nm,流速为1.0mL/min,进样量为20μL,柱温为30℃。结果磺胺嘧啶质量浓度在5～100μg/mL范围内与峰面积有良好的线性关系,r=0.9999(n=5);平均回收率为99.90%,RSD为0.3%。结论HPLC法操作简便、快速,测定结果准确。Objective To establish a HPLC method for the determination of sulfadiazine in Sulfamethoxazole, Sulfaiazine and Trimethoprim Tablet. Methods Using ODS C18 column（150 mm ×4.6 mm, 5 μm）. The mobie phase was water-acetonitrile-triethylamine（799：200 ： 1, adjusting pH to 5.9 by sodium hydroxide reagent or acetic acid glacial）and the detection wavelength was 241 nm, the flow rate was 1.0 mL/min, the injection volume was 20 μL, the colunm temperature was 30℃. Results The linear range was 5- 100 μg/mL, r=0. 999 9 （ n = 5）. The average recovery rate was 99. 90%, RSD = 0. 3%. Conclusion HPLC is convenient, quick and accurate.

关 键 词：磺胺嘧啶 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R978.2