广东地区土壤中分离的棘阿米巴CG/S1株的18Sr DNA基因分析  

Analysis of 18 S rDNA gene of Acanthamoeba sp.CG/S 1 isolated from Guangdong soil

作　　者：王月华[1] 玄英花[2] 郑善子[2] 崔春权[2] 

机构地区：[1]吉林医药学院病原生物学教研室,吉林吉林132013 [2]延边大学基础医学院病原生物学与免疫学教研部,吉林延吉133000

出　　处：《延边大学医学学报》2007年第4期244-246,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]从广东地区土壤中分离棘阿米巴CG/S1株,测定其18 SrDNA基因序列.[方法]从土壤中分离棘阿米巴CG/S1株,提取基因组18 S rDNA,应用棘阿米巴属特异性引物进行PCR扩增,测定序列,用分子生物学软件Clustal X进行序列分析,并与其他棘阿米巴分离株进行比较分析.[结果]棘阿米巴CG/S1的18 S rDNA全基因序列为2 292 bp,基因型为T5型;CG/S1与A.lenticulata7327株的序列差异率为0.61%,与CB/S1株的序列差异率为0.74%.[结论]广东地区土壤中分离的棘阿米巴Acanthamoeba sp.CG/S1为A.lenticulata株.OBJECTIVE To analyze the 18 S rDNA genotype of Acantharmoeba sp, CG/S 1 strains isolated from the Guangdong soil. METHODS The strains of Acanthamoeba sp. CG/S 1 were isolated from soil of the Guangdong. Full 18 S rDNA gene sequences of CG/S 1 were extracted, amplified by using PCR and cloned, then PCR products were sequenced. The complete sequences of 18 S rDNA were analyzed by software Clustal X and used for phylogenetic analysis. RESULTS The full length of CG/S 1 was 2 292 bp and the genotype was T 5. CONCLUSION Acanthamoeba sp. CG/S 1 strains isolated from the Guangdong soil is A. lenticulata strains

关 键 词：基因型 序列分析 棘阿米巴属 

分 类 号：R382.1[医药卫生—医学寄生虫学]