实验性肺血栓栓塞症血红素氧化酶的变化  

Molecular biologic study on heine oxygenase in experiment pulmonary thromboembolism

作　　者：赵鸣雁[1] 罗云鹏[1] 费东生[1] 黄永麟[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院重症医学科,黑龙江150001

出　　处：《中国危重病急救医学》2007年第12期745-748,I0001,共5页Chinese Critical Care Medicine

摘　　要：目的探讨肺血栓栓塞症（PTE）时肺动脉血红素氧化酶（HO）的表达变化，及其对肺动脉压力的影响。方法23只健康成年雄性杂种家犬随机分为PTE3h组（A组）、PTE8h组（B组）、PTE24h组（C组）和对照组（D组）。经右颈静脉置管注入自体血栓建立犬PTE模型，股静脉置入Swan—Ganz导管监测肺血流动力学变化。实验终点处死实验犬取肺动脉，用半定量逆转录-聚合酶链反应（RT—PCR）和免疫组化方法检测HO-1的表达水平。结果各实验组犬肺动脉平均压（PAMP）、肺动脉收缩压（PASP）和肺动脉舒张压（PADP）在栓塞成功后即刻均明显升高，与栓塞前比较差异有统计学意义（P均〈0．05）；栓塞后1h开始均明显下降（P均〈0．05），并呈下降趋势，约4～5h后趋于稳定，栓塞8h点与栓塞前相比仍有升高，但差异无统计学意义。RT-PCR和免疫组化检测结果显示，在D组肺动脉未见HO-1表达；栓塞后各实验组可见HO-1表达，且随着栓塞时间的延长，HO-1表达逐渐增多，以栓塞24h表达量最明显（P均〈0．05）。结论PTE后HO-1表达增加，同时升高的肺动脉压力下降，提示HO-1在PTE时与肺血管舒张相关。Objective To investigate the changes in heme oxygenase （HO） system expression in pulmonary thromboembolism （PTE）, and the influence to the pulmonary artery blood pressure as a result of the changes. Methods In 23 healthy adult male dogs, autologous thrombus was injected through the jugular vein to reproduce the PTE model. They were divided randomly into 4 groups： A （pulmonary embolism 3 hours group）, B （pulmonary embolism 8 hours group）, C （pulmonary embolism 24 hours group） and D （control group）. Swan- Ganz catheter was placed via the femoral vein to observe the changes in hemodynamics. Pulmonary artery was obtained to detect the expression of HO - 1 using semi - quantity reverse transcription-polymerase chain reaction （RT- PCR） and immunohistochemical method. Results The pulmonary artery mean pressure （PAMP）, pulmonary artery systolic pressure （PASP） and pulmonary artery diastolic pressure （PADP） of the experimental groups were increased obviously after the pulmonary embolism as compared with those of before the embolism （all P〈0.05）, and were decreased obviously 1 hour after the embolism （all P〈0.05）. The decrease was gradual and stopped after 4 - 5 hours, but the pressure 8 hours after embolism was still higher than that of before embolism. HO - 1 was not expressed in pulmonary artery in control group, but expressed after embolism in each experimetal group. The expression was increased gradually after pulmonary embolism, and it was remarkable 24 hours after embolism （all P〈0.05）. Conclusion The expression of HO - 1 increased in PTE, and the increased pulmonary artery blood pressure decreased at the same time. The results suggest that HO - 1 is related with dilatation of pulmonary artery after PTE.

关 键 词：肺血栓栓塞症 肺动脉高压 血红素氧化酶 

分 类 号：R686[医药卫生—骨科学]