中国荷斯坦奶牛LOX-1基因3′UTR的PCR-SSCP分析  

Study on the 3′UTR of LOX-1 gene in Chinese Holstein by PCR-SSCP

作　　者：邹荣婕[1] 昝林森[1] 姜向阳[1] 张佳兰[1] 申光磊[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第12期16-20,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十五"科技攻关计划奶类重大专项项目(2002BA518A17);陕西省科学技术研究发展计划项目(2004K01-G1)

摘　　要：为了寻找影响奶牛产奶性状的候选基因,采用PCR-SSCP分子标记方法,对中国荷斯坦奶牛LOX-1基因3′非编码区进行了多态性分析。结果表明,在中国荷斯坦奶牛中检测的LOX-1基因3′UTR有AA型、BB型和AB型3种基因型,其频率分别为0.1374,0.3130和0.5496;不同基因型与中国荷斯坦奶牛的乳脂率之间有显著的相关性(P<0.05),但对乳蛋白率、产奶量和体细胞评分(SCS)影响不大,未达到显著水平。初步认为LOX-1基因可能是控制中国荷斯坦奶牛产奶性能的一个候选基因,或是与之存在紧密遗传连锁的分子标记。In order to detect polymorphisms in the lectin like oxidized low density lipoprotein receptor- 1 （LOX- 1） gene, the polymorphism of LOX- 1 ＇ s 3′ UTR in Chinese Holstein was detected through PCR-SS- CP （Polymerase Chain Reaction-Single Strand Conformation Polymorphism）. The result showed that three genotypes AA,BB and AB of the 3rUTR of LOX-1 gene were found,and the genotype frequency were 0. 137 4,0. 313 0,0. 549 6, respectively in Chinese Holstein. There was highly significant correlation be- tween different genotypes in fat content（P〈0.05）. No significant effect of different genotypes on their ME,protein content and SCS （Somatic Cell Score） was found. These results preliminarily showed that the LOX-1 gene was either a candidate gene that influenced the prolificacy in Chinese Holstein or a molecular marker in close linkage with such a gene.

关 键 词：中国荷斯坦奶牛 LOX-1基因 PCR-SSCP 

分 类 号：S823.912[农业科学—畜牧学]