思茅松胚性愈伤组织的诱导  

Inducement Study of Embryonic Calluses from Pinus kesiya var.langbianensis

作　　者：吴涛[1] 陈少瑜[1] 陈芳[1] 王寅冰[2] 易善军[2] 陈善娜[3] 

机构地区：[1]云南省林业科学院森林植物培育与开发利用重点实验室,云南昆明650204 [2]西南林学院,云南昆明650224 [3]云南大学生命科学学院,云南昆明650091

出　　处：《中南林业科技大学学报》2007年第5期74-78,共5页Journal of Central South University of Forestry & Technology

基　　金：国家林业局948项目"松属树种抗虫基因遗传转化技术引进"(2003-4-44)

摘　　要：以思茅松成熟胚和不同发育时期的未成熟胚为外植体,在DCR、1/2LM和1/2P6培养基上分别添加不同种类及质量浓度组合的植物激素,进行胚性愈伤组织诱导试验.结果表明:一定激素质量浓度范围内的DCR和1/2LM培养基上,成熟胚可以诱导愈伤组织,但都为非胚性愈伤组织;思茅松不同发育时期的未成熟胚在含2,4-D 1.1 mg.L-1、BA 0.45 mg.L-1和KT 0.43 mg.L-1的1/2P6培养基上也可以诱导产生愈伤组织,但只有用处于顶端分生组织原基还不能明显可见时的未成熟胚为外植体进行诱导,才可以得到胚性愈伤组织,诱导率为1.8%;思茅松未成熟球果在普文林场思茅松种子园内最佳采样时期为7月下旬.Calluses were induced from mature zygotic embryos of Pinus kesiya var. langbianensis on DCR and 1/2LM medium supplemented with a certain concentration of 2,4 D, BA, but the induced calluses were non-embryonic ones. Embryonic calluses were initiated from different developmental periods of immature zygotic embryos of the pine on 1/2P6 medium appended with 2, 4-D 1.1 mg. L^-1, BA 0.45 mg. L^-1 and KT 0. 43 mg. L^-1 and its initiation rate was 1. 8%. The optimal time for sampling the immature zygotic embryo in its seed orchard in Puwen Forest Station is the last ten days of July.

关 键 词：遗传育种 思茅松 胚性愈伤组织 诱导 体胚发生 

分 类 号：S791.240[农业科学—林木遗传育种] Q943.1[农业科学—林学]