革兰阴性杆菌中1类整合子的分布与耐药关系  

Relationship between class 1 integron and resistance in gram negative bacteria

作　　者：王寰[1] 范晓磊[2] 

机构地区：[1]大连医科大学第二临床学院北院检验科,辽宁大连116031 [2]大连医科大学微生物学教研室,辽宁大连116044

出　　处：《大连医科大学学报》2007年第6期584-586,共3页Journal of Dalian Medical University

摘　　要：[目的]在革兰阴性杆菌中检测1类整合子,并分析整合子对细菌耐药性的影响。[方法]用VITEK-AMS微生物自动分析仪鉴定细菌和药敏试验,用PCR方法扩增1类整合酶基因,经电泳后检测扩增产物。[结果]266株革兰阴性细菌中检测出1类整合子66株,检出率为24.8%。主要阳性菌为大肠埃希菌34.3%(24/70)、肺炎克雷伯菌48.1%(26/54)、阴沟肠杆菌30%(6/20)、铜绿假单胞菌15.6%(7/45)、鲍曼不动杆菌4.8%(2/42)、产气肠杆菌20%(1/5)。1类整合子阳性菌对氨基糖苷类、喹诺酮类及头孢菌素类药物表现出较高的耐药率。携带1类整合子菌株易表现出对至少4种抗菌素的多重耐药性,其多重耐药率为68.2%(45/66),明显高于1类整合子阴性菌株(28.5%),P<0.05。[结论]1类整合子广泛地存在革兰阴性细菌中,1类整合子对细菌多重耐药性的产生和传播起着重要作用,应加强临床细菌基因水平的耐药监测,采取有效措施防止耐药性的传播。[ Objective] To study the distribution of the class 1 integrons in gram negative bacteria and evaluate the class 1 integrons to the antibiotic resistance. [ Methods ] MICs of 13 different antimicrobial agents against 266 isolates of gram negative bacteria were determined by VITEK GNS card. The presence of class 1 integrase gene was determined by polymerase chain response （PCR） with integrase specific primer and examined by electrophoresis. [ Results] The positive rate of class 1 integron is 24.8% （66/266） in gram negative bacteria. Enterobacter coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa,Acinetobacter baumannii and Enterobacter aerogenes accounted for was 34.3% （24/70）, 48.1% （26/54）, 30% （6/20）, 15.6% （7/45） , 4.8% （2/42） and 20% （1/5） ,respectively. Similarly on the basis of 9 antibiotics in the group with class 1 integrons were higher than that in none - group （P 〈 0.05）. All the integron - positive strains were resistance to at least 4 antibiotics. In the group with class 1 integrons the multi - drugs resistance rate was 68.2% ,but in the none - group was 28.5% ,the difference of the rates between two groups was significant （P 〈 0.05）. Resistant rate of integron - positive strains to aminoglocosides, fluroquinolone and cephalosporins was higher. [ Conclusion] Our study suggests that integron exist in the gram negative bacteria widely. The antibiotics resistance of the integrons positive strains were than higher that of the negative ones.

关 键 词：革兰阴性杆菌 整合子 多重耐药 

分 类 号：R446.5[医药卫生—诊断学]