改性胶原蛋白膜用于大肠杆菌EP8-10组合固定化  

Integrated immobilization of E.coli EP8-10 by modified collagen membrane

作　　者：胡永红[1] 汤天羽[1] 周华[1] 沈树宝[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《生物加工过程》2007年第2期73-76,共4页Chinese Journal of Bioprocess Engineering

基　　金：江苏省自然科学基金资助项目(BK2002017);江苏省高校自然科学基金资助项目(01KJB530003)

摘　　要：探讨了不同方法对胶原蛋白膜进行改性处理,结果表明经过甲醛处理过的胶原蛋白膜具有良好的通透性及耐水性能。将胶原蛋白膜与卡拉胶凝胶颗粒形成组合固定化体系,用于大肠杆菌EP8-10固定化,天冬氨酸转氨酶活回收率达到了91.3%,反应20批次后,酶活回收率仍在80%以上。Different methods related to the modification of collagen membrane were discussed. The colla- gen membrane denatured by formaldehyde had high membrane flux and good water stability. An integrated immobilization was formed with the denatured collagen membrane treated by formaldehyde and gel particles of carrageenan to immobilize E. coli EP8-10 in this research. The recovery of the aspartate aminotransferase activity reached 91.3% by the integrated immobilization matrix, and which was still above 80% after the 20 batches of reactiong.

关 键 词：胶原蛋白膜 组合固定化 大肠杆菌 

分 类 号：Q814.2[生物学—生物工程]