Triton X-100作用下稀土协同发光测定血清中的茶碱  

Determination of Theophylline in Serum by Rare Earth Synergetic Luminescence

作　　者：张珂 黄厚评[1] 黄祖云[1] 蔡汝秀[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《分析化学》1997年第7期825-828,共4页Chinese Journal of Analytical Chemistry

基　　金：国家教委博士点基金资助课题

摘　　要：报道了在表面活性剂 Ｔｒｉｔｏｎ Ｘ－１００存在下，利用Ｇｄ（Ⅲ）离子增敏Ｃｅ（Ⅳ）和茶碱氧化产物的固有发光的协同效应，建立了高灵敏度的测定茶碱的荧光分析方法，其荧光光谱的激发波长λｃｘ＝３２５ｎｍ发射波长λｃｍ＝４０４ｎｍ。在０．ｌ～２０ ｍｇ／Ｌ范围内荧光强度与茶碱浓度呈良好线性关系，其检测下限为０．０５ ｍｇ／Ｌ，方法精密度为２．５％，回收率在９６．０％～１０７．１％，用于血清中痕量茶碱分析取得了满意结果。Great attention has been paid by many researchers to synergetic luminescence as a means of enhancing sensitivity for spectrofluorimetry. Theophylline (i. e. 1,3-dimethylxan- thine) is a bronchodi1ator relaxing agent and respiratory stimulant. In this paper, application of synergetic luminescenece of Gd(Ⅲ) in theophylline assay is presented. Fluorescence of the oxidation product of theophylline oxidized by Ce(Ⅳ) can be greatly enhanced in the presence of non-ionic surfactant (TritonX-100) and gadolinium. The linear range is 0. 1~20mg/L of theophylline and the detection limit is 0. 05mg/L. This method has been satisfactorily ap- plied to the determination of theophylline in serum with recoveries between 96. 0%～107. 1 %.

关 键 词：协同发光 茶碱 稀土 血清 荧光分析 

分 类 号：R974[医药卫生—药品]