高分子控温相变免疫分析法测定苯妥因  

Phase Change Immunoassay of Phenyltoin

作　　者：张柏林[1] 王敏灿[1] 常文保[1] 慈云祥[1] 

机构地区：[1]北京大学化学系,北京100871

出　　处：《分析化学》1997年第9期993-996,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助课题

摘　　要：利用水溶性高分子聚合物Ｎ－异丙基丙烯酰胺和抗体的偶联，用异硫氰酸荧光素标记苯妥因作为竞争物建立了免疫测定苯妥因的新方法，与均相方法相比灵敏度有很大提高，其灵敏度达到 ２５ μｇ／Ｌ，测定可在 ３０ ｍｉｎ内完成。这一新方法可望应用于苯妥因的临床检测。A novel immunoassay of phenyltoin was developed by employing an antibody con- jugated to poly-p-N-isopropylacrylamide (p-NIPAAM ) and phenyltoin-isothio- cyanofluorescein label. The separation in the assay process was achieved by precipitation of poly-p-NIPAAM above its critical solution temperature. The present method is more sensi- tive than previous homogeneous immunoassay of phenyltoin, and the determination can be completed within 30 min with a detection limit of 25 μ g/L.

关 键 词：苯妥因 免疫分析 PRECIPIA 

分 类 号：R971.6[医药卫生—药品]