利用对辣根过氧化物酶催化体系的干扰测定左旋多巴  

Determination of Levodopa by Using the Interference to the Catalytic System of Horseradish Peroxidase

作　　者：朱敏[1] 黄雪梅[1] 李洁 沈含熙[1] 

机构地区：[1]南开大学化学系,天津300071

出　　处：《分析化学》1997年第8期930-933,共4页Chinese Journal of Analytical Chemistry

摘　　要：提出辣根过氧化物酶法分析左旋多巴，方法基于一定条件下左旋多巴对辣根过氧化物酶催化对氯苯酚－４－ＡＡＰ偶联发色体系有干扰，干扰程度与左旋多巴的浓度有相关性。研究了干扰产生的原因和条件，建立了微量左旋多巴的测定方法，检出限可达 ２．５ × １０－７ｍｏｌ／Ｌ。用于左旋多巴片的含量测定，结果令人满意。A method was developed for the enzymatic assay of levodopa. The method is based on the interference to the coupling colorimetric system of p-chloropheno1ic acid and 4- aminoantipyrine catalyzed by horseradish peroxidase (HRP). The resulting change in ab- sorbance at maximum wavelength of coupling product is dependent on the concentration of levodopa. The mechanism of interference was studied preliminarily, and the optimum experi- mental conditions for the measurement were examined. The enzymatic method was used for measuring levodopa with a detection limit of 2. 5 ×107 mol/L. Determination of levodopa in tablet shows excellent correlation with the resu1ts of official methods of the same samples.

关 键 词：辣根过氧化物酶 左旋多巴 酶法分析 

分 类 号：R971.5[医药卫生—药品]