刚果红光度法测定西索米星的含量  

The content determination of sisomicin by congo red spectrophotometry method

作　　者：江虹[1] 湛海粼 

机构地区：[1]长江师范学院化学及环境科学系,重庆408003 [2]涪陵广播电视大学,重庆408000

出　　处：《化学研究与应用》2007年第12期1394-1396,共3页Chemical Research and Application

基　　金：重庆市教委基金资助项目(KJ051304)

摘　　要：In the acid condition of pH 4.5～6.8,congo red(CR) reacts with sisomicin(SISO) to form red ion association complexes and bring to the red solution fading of gongo red.The maximum fading wavelengths were located 504nm,the apparent molar absorptivities(ε) was 2.31×104 L·mol-1·cm-1.The concentration of sisomicin obeys Lombard Beer’s law in 0.10mg/L～6.72mg/L.A new spectrophotometric method for the determination of sisomicin(SISO) has been developed.The optimum reaction conditions and the chief properties of analytical chemistry were studied.The method may was applied to determine the content of the city sell sisomicin and sisomicin in Human urine liquid and Human serum.In the acid condition of pH 4.5 ～ 6. 8, congo red（CR） reacts with sisomicin （SISO） to form red ion association complexes and bring to the red solution fading of gongo red. The maximum fading wavelengths were located 504nm, the apparent molar ahsorpfivifies （ε） was 2.31 ×10^4 L ·mol^-1·cm^-1 The concentration of sisomicin obeys Lombard Beer＇s law in 0. 10mg/L ～ 6.72mg/L. A new spectrophotometric method for the determination of sisomicin （SISO） has been developed. The optimum reaction conditions and the chief properties of analytical chemistry were studied. The method may was applied to determine the content of the city sell sisomicin and sisomicin in Human urine liquid and Human serum.

关 键 词：西索米星 刚果红 分光光度法 

分 类 号：O657.32[理学—分析化学]