高藻胆蛋白钝顶螺旋藻新品系的选育及RAPD分析  

SELECTION AND RAPD ANALYSIS OF A NEW SPIRULINA PLATENSISSTRAIN WITH HIGH PHYCOBILIPROTEINS CONTENTS

作　　者：黄晖[1] 汪志平[1] 张巧生[1] 刘艳辉[1] 李雪斌[1] 徐步进[1] 

机构地区：[1]浙江大学原子核农业科学研究所农业部核农学重点开放实验室,浙江杭州310029

出　　处：《核农学报》2007年第6期567-571,共5页Journal of Nuclear Agricultural Sciences

基　　金：浙江省科技计划重点项目(2007C23052);国家自然科学基金项目(30000010)

摘　　要：测得可用于工厂化生产的9株钝顶螺旋藻的藻蓝蛋白PC、别藻蓝蛋白APC和藻胆蛋白PBP的含量依次为10．74％-16．26％、3．67％-5．55％和14．42％-21．81％，PC与APC的比值在2．87与3．05之间。以PBP含量最高的Sp．CH为出发品系，用组织匀浆破碎和离心沉降法制得其单细胞或原生质球，并先以0．6％EMS处理30mim再用2．4kGy的^60Coγ射线辐照，经藻丝单体分离培养、PBP含量检测及实验室与生产培殖试验，筛选到1株PC、APC和PBP含量依次比Sp—CH的约高36％、89％和50％，PC／APC为1．91～2．23的突变株，命名为Sp—CH32。RAPD分析结果显示，随机引物S38对该突变株与Sp—CH基因组DNA扩增，得到的扩增产物中显示出多态性差异。Sp—CH32经连续3年的工厂化培殖，表现出生产性状好、PBP高产特性稳定，目前已用于大规模养殖与产业化开发。The contents of phycocyanin （PC）, allo-phycocyanin （APC）, phycobiliproteins （PBP） and the ratio of PC and APC （PC/APC） of nine Spirulina platensis strains used in pilot plant cultivation were 10.74% - 16.26% , 3.67%- 5.55% , 14.42% - 21.81% and 2.87 -3.05, respectively. By mechanical grinding and sedimentation, the single cells or spheroplasts of Sp-CH, whose PBP contents was highest in the nine tested strains, were obtained. They were treated by 0.6% EMS for 30min and 2.4 kGy ^60Coγ-rays, and then cultured by the single filament separating, a high PBP content strain named as Sp-CH32 was obtained. The contents of PC, APC and PBP of Sp-CH32 were about 36%, 89% and 50% , higher than that of Sp-CH, respectively. And the PC/APC of Sp-CH32 were 1.91 - 2.23. Moreover, RAPD analysis showed that the amplifcation bands of primers $38 revealed significant polymorphisms in Sp-CH32 and Sp-CH. The pilot plant cultivation in 3 consecutive years showed that Sp-CH32 was a perfect high PBP content strain with excellent characteristics, and has been applied in mass cultivation and industrialization.

关 键 词：钝顶螺旋藻 藻胆蛋白 突变体 育种 RAPD 

分 类 号：S968.4[农业科学—水产养殖]