哮喘儿童痰中炎性细胞及转化生长因子-β_1表达的研究  

Study the inflammatory cells expressing of TGF-β_1 in sputum of children asthma

作　　者：陈强[1] 王江平[2] 刘建梅[1] 柯江维[1] 邹音[1] 

机构地区：[1]江西省儿童医院,330006 [2]浙江省儿童医院

出　　处：《中国妇幼保健》2007年第36期5207-5209,共3页Maternal and Child Health Care of China

基　　金：江西省科技厅社会发展攻关项目(200405)

摘　　要：目的:观察哮喘儿童痰中炎性细胞及转化生长因子-β1(TGF-β1)的表达。方法:负压吸取痰标本39例,其中哮喘组(轻中度哮喘急性发作)婴幼儿24例,非哮喘组(支气管肺炎)婴幼儿15例,同时监测两组患儿的气道阻力。痰标本上清液测白介素-5(IL-5);沉淀物涂片分别行瑞氏染色作普通分类计数及免疫细胞化学测TGF-β1的表达。结果:哮喘组气道阻力为(0.53±0.26)kPa·L-1·S-1明显高于非哮喘组(0.25±0.16)kPa·L-1·S-1,P<0.01;哮喘组嗜酸性粒细胞(Eos)计数为(20.25±15.36)%、IL-5为(16.85±6.54)μg/L与非哮喘组(1.69±2.07)%、(13.56±3.06)μg/L比较,差异有显著性,分别为P<0.01、P<0.05。15例哮喘患儿痰液炎性细胞中出现TGF-β1阳性表达,阳性表达率与Eos计数、IL-5之间呈显著线性相关,分别为(r=0.780,P<0.01),(r=0.892,P<0.01),与气道阻力之间未见明显相关性;非哮喘患儿未见有TGF-β1阳性表达。结论:TGF-β1在哮喘儿童痰中有过度表达,提示TGF-β1与哮喘有关,可为哮喘的诊断提供依据。Objective：To study the inflammatory cells expressing of TGF - β1 in sputum of children asthma. Methods.Sputum specimens of 39 cases were obtainted by vacuum aspiration including asthma group （acute asthma） ： 24 cases, non -asthma group （acute pneumonia） ： 15 cases. The airway interrupter resistance （Rint） of all cases were measured with microloop spirometry devicem. The depositor of sputum specimens was smeared for Eos count and measurement of TGF - β1 expression of inflammtory cells ; and the supernatant was assessed for content of IL -5 with ELISA. Results：The mean Rint was significantly higher in asthma group （0. 53 ±0. 26） kPa/L · S than in non - asthma group （0. 25 ± 0. 16） kPa/L · S （P 〈 0. 01 ）. The Eos count and expression of IL -5 were higher in asthma group than in non - asthma group （P 〈 0. 01, P 〈 0. 05 ） respectively. 15 cases showed positive expression of TGF - β1 of inflammatory cells in sputum of asthma group, there was no positive expression of TGF - β1 in non - asthma group. Forthermore, the ratio of TGF - β1 positive expression showed hnear correlation with Eos count and IL - 5 （ r = 0. 780, P 〈 0.01, r = 0. 892, P 〈 0. 01 ） respectively. There was no relationship with the Rint. Conclusion：The over- expression of TGF- β1 of inflammatory cells in sputum of asthma patients reflects the affection of asthma patient＇s airway, there is relationship between the asthma and inflammatory cells expressing of TGF - β1, which may provide evidences for diagnosing of asthma.

关 键 词：哮喘 儿童 转化生长因子-Β1 免疫细胞化学 痰液 

分 类 号：R722.6[医药卫生—儿科]