榄香烯对过氧化氢损伤血管内皮细胞的保护作用  

The Protection of Elemene on Injured HUVECs Induced by Hydrogen Peroxide

作　　者：王贵学[1] 吴凌艳[1] 唐舒棠[1] 尹铁英[1] 

机构地区：[1]重庆大学生物工程学院,重庆400044

出　　处：《中药材》2007年第11期1407-1410,共4页Journal of Chinese Medicinal Materials

基　　金：科技部国际合作重点项目(2004DFA06400);重庆市科技攻关重大项目(CSTC2006AA5014-3);重庆大学研究生创新实践基地科技创新基金项目(200506Y1B0260133)

摘　　要：目的：探讨榄香烯对内皮细胞增殖的影响和对氧化损伤血管内皮细胞的保护作用。方法：体外培养内皮细胞,将不同浓度的榄香烯（1-200μg/ml）加入到细胞培养液中,用MTT法检测榄香烯对内皮细胞增殖的影响。将1 mmol/L H2O2作用于加入不同浓度的榄香烯预培养24 h的内皮细胞,继续培养1 h,以MDA、T-AOC、SOD、CAT、GSH-Px、NO为检测指标。结果：榄香烯对内皮细胞的增殖呈剂量依赖性的抑制作用,而在抗氧化损伤方面,呈剂量依赖性降低MDA量,降低H2O2对抗氧化酶活力的影响,提高细胞内NO的含量,减少细胞调亡数量。结论：榄香烯可保护内皮细胞结果和功能的完整性,提高内皮细胞抗氧化能力。Objective： To evaluate the effect of elemene on the survival of normal human umbilical endothelial cells（HUVECs） and the protection of elemene on injured HUVECs induced by hydrogen peroxide（H2O2）.Methods： Normal HUVECs were treated with elemene 1~100 μg/ml for 24~72 hours,the survival rate of HUVECs was determined by tetrazolium assay（MTT）.To evaluate the protective effect of elemene on HUVECs from H2O2 injury,HUVECs were injured by 1 mmol/L H2O2 and then different final concentrations of elemene were added before the injury.After culturing 1 hour,then detecting the index of MDA,T-AOC,SOD,CAT,GSH-Px and NO.Results： Elemene could inhabit the proliferation of VEC and it presented dose-dependent, while on the side of anti-oxidization injury,it also presented dose-dependent. MDA content and the effect of H2O2 to antioxidase activity were decreased,NO content in cell was increased,and the amount of apoptosis was reduced.Conclusion： Elemene has dual effects on the survival rate of normal HUVECs in vitro,which is related to the concentration and the duration of drug exposure.Elemene has protective effects on the injured endothelial cells injured by oxidization through the function of anti-lipid oxidization.

关 键 词：榄香烯 内皮细胞 增殖 过氧化氢 细胞损伤 

分 类 号：R285.5[医药卫生—中药学]