超临界耦合常规溶剂法提取新疆雪莲中的总黄酮  

Supercritical Coupling Traditional Way to Extract Flavones in Saussures involucrate

作　　者：张富昌[1] 苏建玲[1] 刘力[1] 赵文军[1] 高林[1] 

机构地区：[1]中国科学院新疆理化技术研究所

出　　处：《中药材》2007年第11期1456-1458,共3页Journal of Chinese Medicinal Materials

基　　金：中国科学院西部行动项目(KGCXZ-SW-510)

摘　　要：通过对新疆雪莲超临界预萃取,然后再用一定浓度的乙醇溶液提取。以总黄酮为指标,运用正交设计法考察了乙醇溶剂的提取温度、时间、液料比、乙醇浓度等因素对总萃取率的影响,与常规溶剂法比较,在各正交条件下总黄酮提取率比常规溶剂法高10.07%-128.4%,并经HPLC指纹图谱验证提取组分基本一致。此法提取效率高,可以降低提取温度,缩短浸出时间,减少有效成分破坏,并能对植物成分实现初级分离（得到2.45%类脂物）。此法扩展了超临界技术在天然产物领域中的应用。The Saussures involucrate was pretreated with supercritical CO2,then the residue was extracted with ethanol.Using a method of orthogonal experiment,the influence of temperature,time,liquid/solid ratios,the concentration of ethanol to the total flavones content were investigated.Compared with traditional extraction method,about more than 10.07%～128.4% flavones were obtained.In conclusion,the supercritical coupling traditional way could enhance the extraction efficiency,lower the extraction temperature,shorten the lixiviating time,reduce the losing of effective component,and pre-extract the plants（obtain about 2.45% oil segment）.The study developed a new application of supercritical technology in the extraction of natural resources.

关 键 词：新疆雪莲 超临界萃取 总黄酮 HPLC 分光光度 正交实验 

分 类 号：R284.2[医药卫生—中药学]