家蝇幼虫抗菌活性物质相关基因表达情况  

The expression of correlative genes of antibacterial materials in housefly larvae

作　　者：渠晖[1] 郝友进[2] 荆迎军[2] 李德森[2] 杜荣骞[2] 

机构地区：[1]南开大学生命科学研究平台,天津300071 [2]南开大学生命科学学院,天津300071

出　　处：《生物加工过程》2007年第4期14-18,共5页Chinese Journal of Bioprocess Engineering

基　　金：天津市自然科学基金资助项目(033604911)

摘　　要：研究了家蝇幼虫体内抗菌活性物质相关基因诱导后的表达情况。选择30%H2O2诱导家蝇幼虫90 min,应用半定量RT-PCR的方法,以组成型表达的β-actin基因作为内参照,对抗菌活性物质基因cecropin,defensin,dipteri-cin,attacin和溶菌酶基因lysozyme在诱导后的表达情况进行了初步探索。Cecropin、defensin和attacin基因在家蝇幼虫体内都是诱导表达型基因,分别在诱导后2 h和6 h达到活性高峰。而diptericin和lysozyme两个基因表达量一直较高,在诱导后6 h表达量达到最大水平,随后开始下降,到诱导后36 h时还能检测到基因有较高水平的表达。The expression of some immune-related genes in housefly （Musca domestica） larvae was investigated. The housefly larvae was induced by 30% H2O2 for 90 minutes, and the expression of antibacterial peptide genes of cecropin, defensin, diptericin, attacin, and lysozyme in transcription level was carried out on semi-quantitive RT-PCR. The transcription of cecropin, defensin and attacin had activity peak at inducing time of 2, 2 and 6 h, respectively. But the transcription devel of diptericin and lysozyme maintained high reached peak after inducing for 6 h.

关 键 词：家蝇 抗菌活性物质 诱导 半定量RT-PCR 

分 类 号：Q75[生物学—分子生物学]