微生物合成11α-羟基-16α,17α-环氧孕酮的动力学  

Kinetics of 11α-hydroxylation of 16α,17α-epoxy progesterone by Rhizopus nigricans

作　　者：郑璞[1] 周英俊[1] 孙志浩[1] 储消和 郭一平 

机构地区：[1]江南大学生物工程学院生物催化研究室工业生物技术教育部重点实验室,无锡214036 [2]浙江升华拜克生物股份有限公司,德清313220 [3]浙江天台药业股份有限公司,天台317200

出　　处：《生物加工过程》2007年第4期44-47,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家973资助项目(2003CB716008);长江学者和创新团队发展计划资助项目(IRT0532)

摘　　要：对耐温黑根霉菌丝体催化16α,17α-环氧孕酮生成11α-羟基-16α,17α-环氧孕酮的反应体系进行了研究,考察了不同反应时间、不同菌体量和底物质量浓度对11α-羟化反应的影响,建立了相应的动力学模型,其方程为r=0.031 5ST1.05+ST。结果表明:提高菌体质量浓度有利于提高反应速率;底物浓度与反应初速率的关系与米氏方程相似。A heat-resistant strain of Rhizopus nigricans was applied to the 11α-hydroxylation of 16α, 17α- epoxy progesterone to produce 11α-hydroxy-16α, 17α-epoxy progesterone. The effects of biomass, reacion time and substrate concentration on the reaction were investigated. The equation was r =0.031Sr/1.05＋Sr The results showed that the reaction rate was higher at higher cell concentration. The relationship between initial rate and substrate concentration can be correlated by the Michaelis-Menten equation and the Km =1.05 g/L,rm was 0. 031 g/（L·Ah）. The model was proposed to describe the initial rate of different substrate concentration.

关 键 词：黑根霉 16α 17α-环氧孕酮 11Α-羟基化 动力学模型 

分 类 号：TQ467.8[化学工程—制药化工]