从酿酒酵母中分离纯化谷胱甘肽  

Separation and purification of glutathione from Saccharomyces cerevisiae

作　　者：林志兴[1] 吕永琴[1] 丁轲[1] 周鑫[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院生物加工过程重点实验室,北京100029

出　　处：《生物加工过程》2007年第4期60-64,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20325622;20576013;50373003);博士点基金资助项目(20030010004);国家"十五"攻关资助项目(2004BA411B05)

摘　　要：为了提高酵母发酵生产谷胱甘肽的提取率,采用热水直接抽提的方法,并通过正交实验优化及单因素实验优化,得到优化条件:鲜酵母的质量与去离子水体积的比例为1∶12;抽提温度90℃;搅拌转速350 r/m in;当抽提液温度达到90℃就停止抽提,并进行了热水抽提的放大实验。同时在抽提时加氮气保护,防止GSH部分氧化。抽提液离心除菌泥,经截流相对分子质量为104的超滤膜超滤后,除去大量杂蛋白,便于后续GSH的精制分离。通过对饱和操作吸附容量及解吸得率的研究,确定强酸型阳离子交换树脂D061为分离介质。Glutathione （GSH） is produced by yeast fermentation. In order to get higher yield of GSH. After centrifugating the broth, the fresh yeast. A new method for extracting glutathione with hot water was studied. Conditions gained with orthogonal experiment were 90 ℃, 12 mL/g, 350 r/min, and stopping extraction as the extraction temperature reached 90 ℃, and a larger scale was proceeded. At the same time it was necessary to protect glutathione with N2 from oxidation in the process. The extraction solution was centrifugated, then ultra-filtrated with membrane of 10 K MWCO （molecular weight cut-off） to remove the impurities of proteins. After this step, the extraction was suitable for the following purification of glutathione. Through the study of total operation exchange capacity and the ratio of desorption, D601 was determined as the separating resin.

关 键 词：谷胱甘肽 正交实验 优化 超滤 离子交换树脂 

分 类 号：TQ028.8[化学工程]