清开灵有效组分调控大鼠脑MVEC缺血再灌注损伤MMP-9mRNA表达的研究  

Effect of QingKaiLing Effective Components on MMP-9mRNA Expression of An in vitro Ischemia and Reperfusion Injury Model of Rat Brain Microvascular Endothelial Cell

作　　者：高永红[1] 牛福玲[1] 朱陵群[1] 李澎涛[2] 王硕仁[1] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室,北京100700 [2]北京中医药大学基础医学院,北京100029

出　　处：《辽宁中医杂志》2007年第12期1806-1808,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展规划项目课题(G1999055404)

摘　　要：目的:观察大鼠脑微血管内皮细胞缺血再灌注损伤MMP-9表达的影响,探讨清开灵有效组分保护血脑屏障阻抑脑缺血再灌注损伤的作用途径。方法:体外培养大鼠脑微血管内皮细胞,氧糖剥夺法模拟缺血再灌注损伤,采用RT-PCR方法半定量检测MMP-9mRNA的表达。结果:与正常组比较,模型组MMP-9mRNA表达明显增加(P<0.01);与模型组比,黄芩苷及新清开灵复方显著降低MMP-9mRNA的表达(P<0.05)。结论:清开灵有效组分可能通过减少血脑屏障内皮细胞MMP-9mRNA表达而阻抑脑缺血损伤炎症反应。Objective ： To observe the expression of matrix metalloproteinase - 9 InRNA of cultured rat brain microvascular endothelial cells （MVEC）, expecting to explore the mechanisms of QingKaiLing effective components protecting brain from injury of inflammatory cascade in cerebral ischemia diseases. Method： Primary culture of rat brain MVEC ,establishing an in vitro ischemia reperfusion model（ OGD）, using RT - PCR method to detect the expression of MMP -9 mRNA. Result： The expression of MMP -9mRNA of model group are significantly higher than normal group（ P 〈0.01 ）, Baicalin and new QingKaiLing injection can depress the expression（ P 〈 0.05 ）. Conclusion： The function of QingKaiLing effective components on inflammatory cascade in cerebral isehemial diseases is probably by intervention of the expression of MMP - 9.

关 键 词：脑微血管内皮细胞 缺血再灌注 基质金属蛋白酶-9 清开灵有效组分 

分 类 号：R285.5[医药卫生—中药学]