益气明目口服液对光化学损伤大鼠视网膜保护作用的研究  

Protective Effect of Yiqimingmu Oral Solution on Rats' Retina with Photochemical Damage

作　　者：唐细兰[1] 王延东[1] 吴伟[1] 叶成添[1] 唐仕波[1] 

机构地区：[1]中山大学中山眼科中心,广州市510060

出　　处：《中国药房》2007年第36期2803-2806,共4页China Pharmacy

基　　金：广东省科技计划项目资助课题(2006B35601001)

摘　　要：目的:研究益气明目口服液对光化学损伤大鼠视网膜的保护作用。方法:48只SD大鼠随机分为阴性对照组、模型组及益气明目口服液高、低剂量组。除阴性对照组外,各组大鼠接受(1 900±106.9)Lux绿色荧光灯24h持续光照射,制备大鼠视网膜光化学损伤模型。益气明目口服液高、低剂量组分别于光照前7d灌胃给药30、15mL.kg-1.d-1;阴性对照组及模型组予以等量生理盐水灌胃。光照后6h、6d、14d检测视网膜组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,并进行视网膜透视电子显微镜和光学显微镜的组织学观察。结果:光化学损伤后6d及14d,益气明目口服液高剂量组视网膜中SOD的活性显著高于模型组(P<0.05),益气明目口服液高、低剂量组视网膜中MDA的含量均显著低于模型组(P<0.05);通过视网膜透视电子显微镜和光学显微镜的组织学观察,益气明目口服液高、低剂量组的病理组织学损害较模型组显著减轻。结论:益气明目口服液对视网膜光化学损伤有显著的保护作用。OBJECTIVE： To observe the protective effect of the traditional Chinese medicine Yiqimingmu oral solution on photochemical damage of retina in rats. METHODS： SD rats were divided into four groups randomly： negative control group, model group, low dose Yiqimingrnu oral solution group and high dose Yiqimingmu oral solution group. All the groups except the control one were continually exposed to Lux （1 900± 106.9） green fluorescent light for 24 hours to establish retinal photochemical damage model. The low dose and high dose groups were administered intragastrically with Yiqimingmu oral solution （15mL · kg^-1 · d^ -1 and 30mL · kg^-1 · d^ -1） 7 days before light expose. Normal saline solution was administered intragastrically in control and model group. At 6 hours and 6, 14 days after light expose, the content of malandialdehyde （MDA） and activity of SOD in rats＇ retina were measured. The histopathologic changes were observed by transmission electron microscopy and light microscopy. RESULTS： At 6 and 14 days after light expose, the activity of SOD in Yiqimingmu solution high dose group were higher than in the model control group（P 〈 0.05）, the contents of MDA in Yiqimingmu solution low and high dose groups were all lower than in the model control group（P 〈 0.05） . Under transmission electron microscope and light microscope, the histopathologic injury of retina in the Yiqimingmu solution low and high dose groups was lessened more than in the model control group. CONCLUSIONS： Yiqimingmu solution has marked protective effect on rats＇ retinal photochemical damage.

关 键 词：益气明目口服液 视网膜 光化学损伤 保护作用 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]