芍药总苷调控巨噬细胞核因子-κB p65蛋白核转移作用的研究  

Regulatory Effect of Total Glucosides of Paeony on Nuclear Translocation of Nuclear Factor-κB p65 in Peritoneal Macrophages

作　　者：贾萍[1] 荣晓凤[1] 丁燕[1] 刘岳凤[1] 田杨[1] 张永如[1] 

机构地区：[1]重庆医科大学附属第一医院中西医结合科,重庆市400016

出　　处：《中国药房》2007年第36期2811-2813,共3页China Pharmacy

摘　　要：目的:研究芍药总苷调控巨噬细胞核因子-κB(NF-κB)p65蛋白核的转移作用,探讨芍药总苷对NF-κB活化的影响。方法:原代培养大鼠腹腔巨噬细胞,经脂多糖和芍药总苷作用后,用Western blot方法观察NF-κB亚基p65蛋白在胞浆和胞核的表达。结果:经脂多糖刺激后,p65蛋白在胞浆中表达显著减少,在胞核中表达显著增多;芍药总苷显著增加了脂多糖刺激后p65蛋白在胞浆中的表达,并减少了其在胞核内的表达。结论:芍药总苷可抑制NF-κB p65蛋白从胞浆向胞核的转移,提示其具有抑制NF-κB活化的作用。OBJECTIVE： To investigate the regulatory effect of total glucosides of paeony on nuclear translocation of nuclear factor- κB （NF - κB） p65 subunit in macrophages and to discuss the its effect on the activation of NF - κB. METHODS： Primarily cultured rats＇ peritoneal macrophages were pre - treated with lipopolysaccharides（LPS） and total glucosides of paeony, then the expression of NF - κB p65 protein in cytoplasm and nuclear in macrophages was determined by western blot analysis. RESULTS： The expression LPS - treated NF - κB p65 protein decreased significantly in cytoplasm but increased significantly in nuclear. Total glucosides of paeony enhanced the expression NF - κB p65 protein in LPS - stimulated cytoplasm but decreased the expression NF- κB p65 protein in LPS - stimulated nuclear. CONCLUSION： Total glucosides of paeony could inhibit the LPS - induced nuclear translocation of NF - κB p65 in rats＇ peritoneal macrophages. This result indicates that total glucosides of paeony may inhibit the activation of NF- κB.

关 键 词：芍药总苷 巨噬细胞核因子-κB 核转移 脂多糖 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]