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机构地区:[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]广东省林业调查规划院,广东广州510500 [3]南京林业大学森林资源与环境学院,江苏南京210037
出 处:《浙江林学院学报》2007年第6期692-695,共4页Journal of Zhejiang Forestry College
基 金:国家自然科学基金资助项目(30371154);"十五"南京林业大学人才基金资助项目
摘 要:为了探索营养储藏蛋白质在杨树体内的积累机制和生理功能,采用凝胶电泳SDS-PAGE,对1年生797杨Populus deltoids×P.euramericana越冬期间皮层和根部中的蛋白质质量分数及营养储藏蛋白质组分进行了测定分析。结果表明,这些部位中的蛋白质在越冬期间质量分数逐渐增加;皮层是杨树越冬期间储藏蛋白质的主要场所。越冬期间储藏蛋白质组分变化最明显的是36,32,22,15 kD等蛋白;落叶时蛋白谱带最清晰,36和32 kD蛋白质是杨树营养储藏蛋白质的主要类型。In order to study the accumulation mechanism and physiological functions of vegetative storage protein (VSPs) in poplar, the contents of proteins and the compound of vegetative storage proteins in the bark and roots during the course of live through winter of the one-year-old poplar clone 797 (Populus deltoids x P. euramericana) were measured and analyzed by SDS-PAGE. The results indicated that the content of proteins in the bark and root increased obviously during winter, and the vegetative storage proteins mainly stored in the bark. The proteins such as 36, 32, 22, 15 kD and etc. changed greatly during the course of through winter, of which these proteins could be detected clearly during the winter and most clear when defoliated. The proteins of 36 and 32 kD are the main types of vegetative storage proteins in poplar. [ Ch, 3 fig. 11 ref.]
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