RT-PCR/Southern杂交法检测穹窿海马伞切割大鼠海马Brn-4 mRNA的表达变化  

Using RT-PCR/Southern blot to detect the change of Brn-4 mRNA expression in rat hippocampus after fimbria fornix transection

作　　者：董传明[1] 秦建兵[1] 金国华[1] 王磊[1] 朱蕙霞[2] 田美玲[1] 

机构地区：[1]南通大学医学院人体解剖学教研室,神经生物学研究所,南通226001 [2]南通大学医学院生物化学教研室

出　　处：《南通大学学报（医学版）》2007年第6期465-467,470,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(30670648);江苏省自然科学基金资助项目(BK2006057)

摘　　要：目的:探讨切割穹窿海马伞大鼠海马与正常海马内Brn-4 mRNA表达的差异。方法:42只SD大鼠随机分成正常对照组和切割穹窿海马伞后1、3、7、14、21及28d组。取各组大鼠的海马组织,提取总RNA,采用RT-PCR/Southern杂交方法分析穹窿海马伞切割后海马内Brn-4 mRNA的表达变化。结果:海马内Brn-4 mRNA的表达量在切割后第3d开始升高,14d达到最高水平,随后下降,28d左右恢复至正常水平。结论:切割穹窿海马伞后海马内Brn-4mRNA表达明显上调,可能与促进海马内移植的或自体的神经干细胞向神经元分化有关。Objective： To investigate the difference of Brn-4 mRNA expression between the fimbria fomix transected rats and normal ones. Methods： 42 SD rats were randomly divided into 7 groups, 6 rats in each group.One group served as normal control and the others served as the 1st, 3rd, 7th , 14th , 21st and 28th day group after fimbria fomix transection, respectively. Then hippocampi were isolated and total RNA was extracted. RT-PCR/Southem blot was used to detect the change of Brn-4 mRNA expression in hippocampus after fimbria fomix transection. Results： The expression level of Brn-4 mRNA started to increase on the 3rd day after fimbria fornix transection.The peak appeared on the 14th day,and then the expression decreased a, slowly to pre-transecflon level on the 28th day. Conclusion： The process that the expression of Brn-4 mRNA increases significantly after fimbria fomix transection, perhaps, might be related to the transplanted or endogenous neural stem cells differenflaring into neurons in hippocampus.

关 键 词：穹窿海马伞切割 海马 Brn-4 逆转录聚合酶链反应 SOUTHERN杂交 大鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]