血清饥饿释放过程中Raji细胞内Skp2与p27^(kip1)的表达变化及意义  

Expression variation and significance of Skp2 and p27^(kip1) during serum starvation and release process of Raji cells

作　　者：邵苏吉[1] 陆建新[2] 王燏婵[2] 沈爱国[2] 赵玥铭[2] 张冬梅[2] 程纯[2] 

机构地区：[1]南通大学附属医院输血科,南通226001 [2]南通大学医学院微生物与免疫学教研室

出　　处：《南通大学学报（医学版）》2007年第6期474-475,478,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省高校自然科学研究项目(04KJB320114);江苏省卫生厅资助项目(H200632);江苏省南通市社会发展项目(S2006003)

摘　　要：目的:探讨血清饥饿释放过程中淋巴瘤细胞株Raji细胞内Skp2与p27kip1的表达变化及意义。方法:采用免疫沉淀方法检测Raji细胞中p27kip1与Skp2的结合情况;采用血清饥饿合并释放同步化处理Raji细胞,经WesternBlot技术检测该过程中p27kip1、Skp2在Raji细胞中的表达变化。结果:免疫沉淀结果显示在Raji细胞中p27kip1与Skp2能够相互结合。在Raji细胞血清饥饿释放过程中,Skp2蛋白总量增加,p27kip1蛋白总量减少。结论:在Raji细胞血清饥饿释放过程中Skp2合成增加,可能通过加快p27kip1的泛素化降解而使细胞中p27kip1显著减少,从而推动了细胞周期的进程。Objective： To investigate the expression variation and significance of Skp2 and p27kip1 during serum starvation and release process of Lymphoma cell line Raji cells. Methods： The combination of p27kip1 and Skp2 were detected by Immuno- precipitation in Raji cells. Raji cells were treated by serum starvation and release assay for synchronization purpose. The ex- pression variation of p27kip1, Skp2 were detected by Western blot. Results： The results of immunoprecipitation suggest p27kip1 and Skp2 could form compound in Raji cell. During serum starvation and release process of Raji cells, the albumen amount uf Skp2 increased, while the albumen amount of p27kip1 decreased, Conclusion.. During serum starvation and release process of Raji cells, the increased synthesis of Skp2 may speed up p27kip1 degradation via the ubiquitin-proteasome pathway, then p27kip1 decreased significantly, and then the cell cycle was put forward.

关 键 词：RAJI细胞株 P27KIP1 S期激酶相关蛋白2 

分 类 号：Q786[生物学—分子生物学]