利多卡因对缺氧/复氧培养大鼠心肌细胞c-fos表达及LDH释放的影响  

The effect of lidocaine on the expression of c-fos and LDH of cultured neonational rat cardiomyocytes subjected to hypoxia/reoxygenation

作　　者：唐标[1] 黄新冲[1] 

机构地区：[1]南通大学附属医院麻醉科,南通226001

出　　处：《南通大学学报（医学版）》2007年第6期489-490,493,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:研究利多卡因对缺氧/复氧培养大鼠心肌细胞LDH漏出及c-fos表达的影响。方法:建立SD大鼠心肌细胞原代培养及缺氧/复氧模型。将40孔原代培养大鼠心肌细胞分为5组,每组8孔。Ⅰ组为对照组;Ⅱ组缺氧2h复氧3h;Ⅲ、Ⅳ和Ⅴ组与Ⅱ组比在缺氧前开始分别加入利多卡因50μmol.L-1、100μmol.L-1和150μmol.L-1。复氧3 h时用生化比色法测定培养基中乳酸脱氢酶(LDH)活力。将75孔细胞同上分为5组,在复氧2 h时用免疫组化法和图像平均灰度分析法测定c-fos的表达。结果:3种浓度利多卡因均能减少心肌细胞LDH漏出(P<0.05)。Ⅱ组与Ⅰ组比c-fos表达明显增加,Ⅵ组和Ⅴ组与Ⅱ组比较c-fos表达明显被抑制,平均灰度值差异有统计学意义(P<0.05)。结论:3种浓度的利多卡因能防治心肌细胞缺氧/复氧损伤,并且呈剂量相关性。Objective： To observe the effect of lidocaine on c-los expression in cultured cardiac myocytes of neonatal rats and the concentration of lactate dehydrogenase（LDH） in culture medium during reoxygenation. Methods： Original cultured cardiomyocytes of SD rat and hypoxia-reoxygenation injuried immature cariomyocyte were established. The 40 caves of cultured cardiac myocytes were divided into 5 groups： group Ⅰ as control;group Ⅱ as hypoxia-reoxygenation （hypoxia 2 hours-reoxygenation 3 hours）control; With eomparaded to group Ⅱ ,the groups Ⅲ、Ⅳ, Ⅴ and Ⅵ were treated with lidocaine（50 μmol·L^-1, 100 μmol·L^-1 and 150 μmol·L^-1） since hypoxia respectively. Immunohistochemistry and mean gray were used to estimate the effect of lidocaine on expression of c-los in cultured rat cardiomyocyte of each group 2 hours after reoxygenation. Release of lactate dehydrogenase of the cardiomyocytes of each group was investigated and determined as injury index of cardiomyocytes. Results： Release of lactate dehydrogenase was inhibited by lidocaine in 3 kinds of density,and expression of c-los of the cardiomyocytes was significantly inhibited by lidocaine in 100 μmol·L^-1 and 150 μmol ·L^-1 （O〈0.05） .Conclusion： Hypoxia-reoxygenation injury of the cardiomyocyte was released, then the expression of c-fos during reoxygenation was inhibited by lidocaine.

关 键 词：心肌细胞 缺氧/复氧 c—fos 利多卡因 

分 类 号：R971.2[医药卫生—药品]