两种内分泌干扰物对鲫鱼淋巴细胞活性与功能的影响  

Effect of two endocrine disruptors on lymphocyte growth and proliferation of fish crucian carp(Carassius auratus)

作　　者：丁辉[1] 伊雄海[1] 陆贻通[1] 

机构地区：[1]上海交通大学农业与生物学院,上海201101

出　　处：《环境污染与防治》2007年第12期881-884,共4页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No20577033);国家重点基础研究发展规划资助项目(No2004CB418503)

摘　　要：从鲫鱼的血样中分离淋巴细胞,以内分泌干扰物甲草胺和莠去津染毒,体外培养48h,采用四甲基偶氮唑盐(MTT)比色法测定两种除草剂对淋巴细胞生长活性的影响,并进行淋巴细胞的增殖实验,确定植物血凝素(PHA)刺激淋巴细胞增殖的适宜浓度,在此基础上开展两种除草剂对鲫鱼淋巴细胞增殖影响的研究。结果表明,甲草胺和莠去津对鲫鱼淋巴细胞生长活性的影响呈剂量—效应关系,其半数抑制浓度(IC50)分别为(27.7±7.6)、(97.6±26.4)μg/L;PHA的适宜刺激质量浓度是10.0μg/mL;两种除草剂对鲫鱼淋巴细胞增殖存在影响,当甲草胺质量浓度大于7μg/L、莠去津质量浓度大于50μg/L时,淋巴细胞增殖受到明显抑制,表明其对鲫鱼存在潜在的免疫毒性。The lymphocyte was separated from the blood sample of the fish Carassius auratus and cultured in vitro for 48 hours under exposure to alachlor and atrazine. The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）method was used to determine the optimum concentration of phytohaemoagglutinin（PHA）in the pro- liferation test and the effects of alachlor and atrazine on lymphocyte growth and proliferation. The optimum PHA concentration was 10.0 μg/mL. Both alachor and atrazine affected lymphocyte growth, exhibiting the half inhibition concentration（IC50）of （27.7±7.6） μg/L and （97.64±26.4） μg/L,respectively. They also affected lymphocyte proliferation; the proliferation was significantly inhibited when the alachlor concentration was above 7 μg/L or the atrazine concentration was above 50 μg/L. The experimental results demonstrated that alachlor and atrazine have potential immunotoxicity to Carassius auratus.

关 键 词：甲草胺 莠去津 淋巴细胞增殖 MTT比色法 

分 类 号：X503.225[环境科学与工程—环境工程]