庆大霉素损伤后豚鼠前庭上皮STAT3 mRNA的表达变化及意义  

Expression changes and biological significance of STAT3 mRNA during self-repair in vestibular epithelium of guinea pigs after gentamycin toxicity

作　　者：盛宏申[1] 徐光[1] 孙景豫[2] 何跃[1] 王锦玲[3] 

机构地区：[1]成都军区总医院耳鼻喉科,四川成都610083 [2]成都军区昆明总医院耳鼻喉科,云南昆明650032 [3]第四军医大学西京医院耳鼻喉科,陕西西安710032

出　　处：《西南国防医药》2007年第6期679-682,F0004,共5页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金资助项目(39870788)

摘　　要：目的:观察庆大霉素损伤后豚鼠前庭上皮修复期细胞增殖和STAT3 mRNA的表达变化情况,探讨其生物学特性和意义。方法:采用原位杂交组织化学方法,检测庆大霉素损伤后豚鼠前庭上皮STAT3 mRNA的表达变化;采用Brdu体内标记和抗Brdu抗体免疫组化染色,检测庆大霉素损伤后豚鼠前庭上皮修复期细胞增殖情况。结果:在正常成年豚鼠前庭上皮中,STAT3 mRNA有低水平表达。庆大霉素损伤后1 d组,STAT3 mRNA杂交信号最强,其后逐渐减少,21d组与正常对照组无显著差异。在正常对照组前庭上皮中无Brdu阳性细胞。各实验组均观察到Brdu阳性细胞,7 d组最多。结论:庆大霉素损伤后,豚鼠前庭上皮STAT3 mRNA表达增强,其时程变化与细胞增殖活动密切相关。STAT3在豚鼠前庭上皮损伤后的自发修复中可能起重要信号转导作用。Objective：To evaluate the biological characteristics and roles of STAT3 during hair cell self - repair, the expression of STAT3 mRNA and cell proliferation of vestibular epithelium in guinea pigs after gentamicin toxicity were investigated. Methods： The expression of STAT3 mRNA was examined by in situ hybridization. Cell proliferation was detected by immunohistochemical anti - Brdu. Results： In the control group, the expression of STAT3 mRNA was low in vestibular epithelium. Whereas, the level of STAT3 mRNA reached significantly to its maximum in the 1 d group and then decreased gradually. Brdu labeled cell was not detected in the control group. But in the experimental groups, Brdu positive cells were observed and significantly increased in the 7 d group. Conclusion： The expression of STST3 mRNA of vestibular epithelium in guinea pigs increased after gentamicin toxicity. Furthermore, the time course of the expression of STAT3 mRNA correlated with the proliferation of vestibular epithelial cells. Therefore, STAT3 may play an important role in signal transduction during hair cell self - repair in guinea pigs after gentamycin toxicity.

关 键 词：STAT3 前庭上皮 细胞增殖 庆大霉素 

分 类 号：R764.83[医药卫生—耳鼻咽喉科] Q752[医药卫生—临床医学]