桑树根际解磷细菌的分离鉴定及解磷能力测定  

Isolation,Identification and the Phosphate Solubilizing Capacity of Mulberry Rhizosphere Phosphbacteria

作　　者：吴凡[1] 崔萍[1] 夏尚远[1] 杨少波[1] 辛磊[1] 刘训理[1] 

机构地区：[1]山东农业大学林学院

出　　处：《蚕业科学》2007年第4期521-527,共7页ACTA SERICOLOGICA SINICA

基　　金：山东省科技攻关项目(编号02201010102)

摘　　要：桑树根际解磷细菌能够改善桑树营养,促进根系生长。利用选择性培养基,从桑树根际分离获得32个具有解磷能力的细菌分离物,经rep-PCR基因指纹分析得到19株解磷细菌。经解磷能力测定,PYP2、PYP4、PYP6、FYP2菌株具有较强的解有机磷能力,FWP7、PWP4菌株具有较强的解无机磷能力,FWP1、FWP9、FWP11、PYP6和PWP1菌株的解有机磷和无机磷能力均较强。利用菌落形态特征观察及16S rDNA碱基序列测定和同源性分析,对具有较高解磷能力的菌株进行了鉴定:FWP1、FWP11、PYP2、PYP6、FYP2菌株为假单胞菌属(Pseudomonas sp.),FWP9和PWP1菌株为贪噬菌属(Variovorax sp.),FWP7、PWP4菌株为根瘤菌属(Rhizobium sp.)。The phosphbacteria of murberry rhizosphere were isolated and cultivated by the plate cultivation method with selective media, and the phosphate solubilizing capacities of those strains were investigated. The strains that had high phosphate solubilizing capacity were identified based on the results of phenotypic characteristics and phylogenetic analysis of 16S rDNA sequences. Nineteen phosphbacteria strains were obtained from mulberry rhizosphere. The strains of PYP2, PYP4, PYP6, FYP2 had high organic phosphate solubilizing capacity. The strains of FWP7 and PWP4 had high inorganic phosphate solubilizing capacity. The strains of FWP1, FWP9, FWP11, PYP6 and PWP1 had organic phosphate solubilizing capacity and inorganic phosphate solubirizing capacity. FWP1, FWP11, PYP2, PYP6, and FYP2 beronged to Pseudomonas sp. FWP9 and PWP1 belonged to Variovorax sp. FWP7 and PWP4 belonged to Rhizobium sp.

关 键 词：桑树 解磷细菌 rep-PCR基因指纹分析 解磷能力 

分 类 号：S888[农业科学—特种经济动物饲养]