桑叶多糖的分离及红外光谱和气相色谱分析  

Infrared Spectrum and Gas Chromatogram Analysis of the Polysaccharides Separation From Mulberry Leaves

作　　者：张剑韵[1] 包立军[1] 梁进[1] 黄龙全[2] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽农业大学茶与食品科技学院,合肥230036

出　　处：《蚕业科学》2007年第4期549-552,共4页ACTA SERICOLOGICA SINICA

基　　金：安徽省教育厅自然科学研究项目(编号2003KJ126);安徽省教育厅自然科学重点科研项目(编号2005KJ393ZD)

摘　　要：多糖是桑叶中的重要功能性成分之一。采用水煮提取、乙醇分级沉淀和Sephadex G-100凝胶层析的方法,从桑叶中分离、纯化出3种多糖组分:MPSⅠ、MPSⅡ和MPSⅢ。经红外光谱分析,MPSⅠ不同于MPSⅡ和MPSⅢ,表现出长分子链、β-糖苷键构型和含酰胺基团等特征。气相色谱分析结果显示,L-鼠李糖、D-葡萄糖和D-半乳糖是3种桑叶多糖的共同成分,其中D-葡萄糖和D-半乳糖的构成比例较大,3种桑叶多糖的单糖构成也存在明显差异。Three kinds of polysaccharides （MPSⅠ , MPSⅡ, MPSⅢ） were separated from the water extract of mulberry leaves, by fractional precipitation with ethanol concentrations at 30%, 50% and 80% respectively, and further purified by sephadex G-100 gel column chromatography. Compared with the other two polysaccharides, MPSⅠ charaeterized as β-glycosidic bond, a longer molecule chain and a kind of glycosaminoglycan by infrared spectrum analysis. Gas chromatogram results showed that L-rhamnose, D-glucose and D-galactose are the common components, and D-gLucose and D-galactose are the major monosaccharide in the mulberry polysaccharides. Monosaccharide constitution also showed obvious difference among the three kinds of mulberry polysaccharide.

关 键 词：桑叶多糖 分离 红外光谱 气相色谱 

分 类 号：S888[农业科学—特种经济动物饲养]