金柑LEAFY同源基因克隆与全序列分析  

Cloning and sequence analysis of LEAFY homologous gene from kumquat(Fortunella crassifolia Swingle)

作　　者：何新华[1] 郭永泽[1] 张利[1] 李杨瑞[2] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西农业科学院作物遗传改良与生物技术重点实验室,广西南宁530007

出　　处：《广西农业生物科学》2007年第4期273-276,共4页Journal of Guangxi Agricultural and Biological Science

基　　金：国家自然科学基金(30560007);广西自然科学基金(桂科自0542022);广西科学基金应用基础研究专项(桂科基0731040)

摘　　要：通过PCR扩增,从融安金柑基因组DNA中克隆出一条2 361 bp的DNA片段,该DNA克隆含有1个2 081 bp的LEAFY同源基因全长序列(FcLFY)。金柑LEAFY同源基因包含2个内含子和3个外显子,编码398个氨基酸。比对结果表明,金柑LEAFY同源基因与甜橙、枳的LEAFY同源基因的核苷酸和氨基酸序列的同源性均为98%。该研究为今后从分子水平上研究金柑开花调控机理打下了坚实的基础。A 2 361 bp DNA fragment was isolated and cloned by polymerase chain reaction （PCR） from kumquat （Fortunella crassifolia Swingle）. The DNA clone contained a complete sequence of 2 081 bp LEAFY homologous gene （FcLFY） which included 2 introns and 3 exons and encoded 398 amino acids. The alignments of nucleotide and amino acid sequences of their LEAFY homologous genes between kuamquat with sweet orange and Poncirus trifoliata were all 98%. The research laid a sound foundation for studying the regulation and control in flowering mechanism in kumquat at the molecular level in the future.

关 键 词：金柑 LEAFY 克隆 

分 类 号：S666[农业科学—果树学] Q781[农业科学—园艺学]