地锦叶片正丁醇提取物的抑菌作用  

Antimicrobial Activity of n-butanol Extract from Parthenocissus tricuspidata Leaves

作　　者：刘华玲[1] 马欣荣[1] 孙振元[2] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2007年第6期872-875,共4页Forest Research

基　　金：国家"863"资助项目(2001AA244031);地锦种质资源创新及优良品种培育

摘　　要：The n-butanol extract from Parthenocissus tricuspidata leaves were examined for antimicrobial activity against five microbes by using filter paper method.The results showed that the extract restrained the growth of Escherichia coli,Agrobacterium tumefaciens,Bacillus subitilis and Bacillus pumilus,while inhibited the growth of yeast at a certain extent.And the minimum inhibitory concentrations(MIC) were 0.25,0.3,0.25,0.3,1.0 g·mL-1respectively.Moreover the n-butanol extract also could restrained some bacterium strains,which had antibiotic resistances.After heated at 95 ℃ for 2～3 h,the n-butanol extract from P.tricuspidata leaves still presented strong bacteriostasis to germs.This result indicated that the substance had heat-stability.The n-butanol extract from Parthenocissus tricuspidata leaves were examined for antimicrobial activity against five microbes by using filter paper method. The results showed that the extract restrained the growth of Escherichia coli ,Agrobacterium tumefaciens ,Bacillus subitilis and Bacillus pumilus ,while inhibited the growth of yeast at a certain extent. And the minimum inhibitory concentrations （MIC） were 0.25,0.3,0.25,0.3,1.0 g · mL^- 1 respectively. Moreover the n-butanol extract also could restrained some bacterium strains, which had antibiotic resistances. After heated at 95 ℃ for 2-3 h, the n-butanol extract from P. tricuspidata leaves still presented strong bacteriostasis to germs. This result indicated that the substance had heat-stability.

关 键 词：地锦叶片 正丁醇提取液 抑菌作用 最低抑菌浓度(MIC) 

分 类 号：R282.71[医药卫生—中药学]