硫酸镍对大鼠睾丸生精细胞c-Fos和HSP70 mRNA表达的影响  

Effects of nickel sulfate on the c-Fos and HSP70 expression of testicular spermatogenic cells in rats

作　　者：孙应彪[1] 王学习[1] 刘一亚[1] 朱玉真[1] 

机构地区：[1]兰州大学公共卫生学院,甘肃兰州730000

出　　处：《毒理学杂志》2007年第5期383-386,共4页Journal of Toxicology

摘　　要：目的观察硫酸镍（NiSO4）对大鼠睾丸生精细胞c—Fos和HSP70mRNA表达的影响。方法健康性成熟Wistar雄性大鼠32只，随机分为4组：正常对照组（Ns组）、NiSO4 1．25、2．5、5．0mg／kg组。腹腔注射染毒，1次／d，连续30d。采用原位杂交技术检测大鼠睾丸生精细胞c—Fos和HSP70 mRNA的表达水平。结果NiSO4 2．5、5．0mg／kg组睾丸精原细胞c-Fos mRNA阳性表达细胞数减少，主要见于生精周期Ⅰ～Ⅵ期、Ⅻ～ⅩⅢ期和ⅩⅣ期；精母细胞HSP70 mRNA阳性表达细胞数明显增多，主要见于生精周期Ⅰ～Ⅵ期和Ⅻ～ⅩⅢ期。结论NiSO4引起大鼠睾丸生精过程障碍可能与其所致的精原细胞c—Fos mRNA表达下调和精母细胞HSP70 mRNA表达上调有关。Objective To explore the effects on the c-Fos and protein 70(HSP70)mRNA expression of testicular spermatogenic cells induced by nickel sulfate(NiSO_4).Methods The male Wistar rats with sexual maturation were divided into four groups randomly,and three groups of rats were administrated intraperitoneally with NiSO_4 at doses of 1.25,2.50,5.00 mg/kg respectively for 30 d.The expression of c-Fos and heat shock HSP70 mRNA of seminiferous epithelium cells were detected through in situ hybridization techniques.Results The c-Fos mRNA expression of spermatogonia significantly decreased at the Ⅰ～Ⅵ,Ⅻ～ⅩⅢ and ⅩⅣ stages of the seminiferous epithelium cycle and the HSP70 mRNA expression of spermatocyte significantly increased at the Ⅰ～Ⅵ,Ⅻ～ⅩⅢ stages of the seminiferous epithelium cycle than that of control group in NiSO_4 2.50,5.00 mg/kg groups(P<0.05).Conclusion The abnormal changes of testicular spermatogenesis induced by NiSO_4 may be partly related to the downwards expression of c-Fos in spermatogonia and the upward expression of HSP70 in spermatocyte.

关 键 词：硫酸镍(NiSO4) 生精细胞 c—Fos HSP70 

分 类 号：R994.3[医药卫生—毒理学]