肿瘤坏死因子α基因多态性与老年2型糖尿病的相关性研究  

The Relationship of Polymorphism of TNF-α and Type 2 Diabetes Mellitus in Elderly Patients

作　　者：饶高峰[1] 陈恩富[1] 曾爱平[1] 林海洋[1] 吴金友[1] 

机构地区：[1]温州医学院附属温岭医院,317500

出　　处：《医学研究杂志》2007年第12期69-70,共2页Journal of Medical Research

摘　　要：目的研究肿瘤坏死因子α(TNF-α)基因多态性与老年2型糖尿病的相关性。方法利用DNA测序法对60例老年2型糖尿病患者及30例正常对照者的TNF-α基因-308位点和-238位点进行基因多态性分析。结果TNF-α基因-308位点在患病组中变异的G/A基因型频率为21.7%,与对照组组间比较有高度显著差异(P<0.01),而-238位点在两组之间无显著性差异(P>0.05)。结论TNF-α基因G-308A单核苷酸多态性与老年2型糖尿病可能存在相关性。Objective To study the relationship of TNF - α G308A and G238A polymorphism with type 2 diabetes mellitus in elderly patients. Methods Blood specimens were collected from 60 elderly patients with type 2 diabetes mellitus and 30 normal elderly volunteers. Genomic DNA was extracted. The primers of PCR for the SNPs of G308A and G238A were optimized and the polymorphism of amplicons was analyzed by DNA sequencing. Results There was a significant difference of TNF - α - 308 gene in DM patients compared with control group, while no difference of TNF - α - 238. Conclusions The 308 G - A polymorphism of TNF - α promoter gene may be associated with type 2 diabetes mellitus in elderly patients.

关 键 词：老年人2型糖尿病 肿瘤坏死因子 单核苷酸多态性 

分 类 号：R587.1[医药卫生—内分泌]