嗅三针电刺激对急性局灶性脑缺血再灌注大鼠大脑皮层组织过氧化氢酶、谷胱甘肽过氧化物酶和总抗氧化能力的影响  

Effects of Electroacupuncture on Cerebro-cortical Catalase,GSH-Px and Total Anti-oxidation Capability in Focal Cerebral Ischemia-reperfusion Rats

作　　者：牛文民[1] 刘智斌[1] 

机构地区：[1]陕西中医学院,咸阳712083

出　　处：《针刺研究》2007年第6期377-379,共3页Acupuncture Research

摘　　要：目的:探索嗅三针电刺激干预急性局灶性脑缺血再灌注大鼠病理损伤的作用机制。方法:30只SD大鼠随机分为假手术组、模型组和观测组,每组10只。采用线栓法制作大鼠急性脑缺血再灌注模型。电刺激嗅三针治疗观测组采用平刺进针,电针选用疏密波,频率80～100Hz,电流1～3mA,电压1～3V,留针60min。测定3组大脑皮层组织过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)。结果:模型组大脑皮层组织CAT、GSH-Px活性和T-AOC均明显低于假手术组,二者相比差异有极显著性意义(P<0.01);观测组经嗅三针电刺激治疗后,大脑皮层组织CAT、GSH-Px活性和T-AOC显著升高,与模型组相比差异有极显著性意义(P<0.01)。结论:嗅三针电刺激可提高急性局灶性脑缺血再灌注大鼠皮层组织CAT、GSH-Px活性和T-AOC,这些作用可能是嗅三针电刺激改善脑缺血再灌注脑损伤的机制之一。Objective： To observe the effect of electroacupuncture （EA） of “Xiusanzhen” on anti-oxidation in cerebral ischemia-reperfusion （CI-R） injury rats and to analyze its underlying mechanism in resisting cerebral ischemic injury. Methods： A total of 30 SD rats were randomized into sham, model and EA groups, with 10 cases in each. CI-R model was established by re- versible occlusion of the middle cerebral artery. EA （80-100 Hz, 1- 3 mA） was applied to “Xiusanzhen” （the mid-point of the nasal root, 2 mm bilateral to the nasal root） for 60 min. The levels of catalase （CAT）, glutathione peroxidase （GSH-Px） and total anti-oxidation capability （T-AOC） in cerebral cortex tissue were determined by using spectrophotometry and chromatometry re- spectively. Results： Compared with control group, the levels of CAT, GSH-Px and T-AOC in model group were markedly lower （P〈0.01）;while compared with model group, the levels of CAT, GSH-Px and T-AOC increased significantly in EA group （ P〈0.01 ）. Conclusion： EA of “Xiusanzhen” has definite effects in raising CI-R-induced decrease of CAT, GSH-Px activity and T-AOC in the cerebral cortex, which may contribute to its effect in reducing ischemic cerebral damage.

关 键 词：脑缺血再灌注 嗅三针 电刺激抗氧化 

分 类 号：R245.32[医药卫生—针灸推拿学]