针刺对单纯性肥胖大鼠血清瘦素含量和下丘脑瘦素受体表达的影响  

Influence of Acupuncture on Serum Leptin Level and Hypothalamic Leptin Receptor Expression in Simple Obesity Rats

作　　者：杨春壮[1] 马英[1] 徐永良[1] 王羽[1] 王莹[1] 张大伟[1] 

机构地区：[1]牡丹江医学院,牡丹江157011

出　　处：《针刺研究》2007年第6期384-388,共5页Acupuncture Research

基　　金：黑龙江省教育厅科学技术研究项目(11511431)

摘　　要：目的:观察针刺对肥胖大鼠血清瘦素含量和下丘脑瘦素受体表达的影响,探讨针灸减肥的机制。方法:Wistar雄性大鼠45只,随机分为正常对照组、模型组和针刺组,各15只。用高脂饲料喂养制作肥胖大鼠模型。针刺大鼠"后三里"和"中脘"穴,并接通电针仪,频率10Hz,电压1.5V,每次治疗10min,每日1次,连续治疗14d。每10d测体重、体长并计算Lees指数。放射免疫法检测血清瘦素含量,免疫组织化学法检测下丘脑瘦素受体表达。结果:针刺组血清瘦素含量比模型组降低(P<0.01),下丘脑瘦素受体表达增加(P<0.01);针刺组血清瘦素水平、下丘脑瘦素受体表达与正常组比较差异仍有显著性意义(P<0.05,0.01)。结论:肥胖大鼠瘦素抵抗状态的改善是针刺减肥的作用机制之一;针刺减肥需要一定的时程。Objective： To observe the effect of electroacupuncture （EA） on serum leDtin contents and the expression of leptin receptor in the hypothalamus in simple obesity rats so as to explore the underlying mechanism of acupuncture in reducing weight. Methods ： A total of 45 male Wistar rats were randomly divided into normal control, model and EA groups with 15 cases in each. Obesity model was established by feeding the animal with high fat forage. EA （10 Hz, 1.5 V） was applied to “Zusanli” （ST 36） and “Zhongwan” （CV 12） for 10 min, once daily for 14 days. The body weight, body length and Lee＇s index were measured once every 10 days. Serum leptin contents were measured by radioimmunoassay, and leptin receptor expression was detected with immunohistochemistry. Results： In comparison with normal control group, the body weight, Lee＇s index and serum leptin content of model group increased significantly （ P〈0.05, 0.01 ）, while in comparison with model group, these 3 indexes in EA group decreased markedly （P〈0.05, 0.01）. Compared with normal control group, the number of leptin receptor immuno-reac-tion （IR）-positive cells in the hypothalamus in model group decreased considerably （P〈0.01）, while compared with model group, the number of IR-positive cells increased significantly in EA group （P〈0.01）. It suggests that EA-stimulation induced im- provement of leptin resistance may contribute to its effect in reducing body weight. Cenclusion： EA can lower serum leptin level and upregulate the expression of leptin receptor in the hypothalamus in obesity rats.

关 键 词：肥胖 血清瘦素 下丘脑 瘦素受体 

分 类 号：R245.97[医药卫生—针灸推拿学]