HIL-29通过激活MAPK信号途径上调Hela细胞TLR3表达  

HIL-29 up-regulate the expression of Toll-receptor 3 in Hela cell involved MAPK activated

作　　者：罗欣[1] 黄海良[1] 陈兵[1] 张胜权[1] 

机构地区：[1]安徽医科大学生物化学与分子生学教研室,安徽合肥230032

出　　处：《中国生化药物杂志》2007年第6期369-371,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：安徽省自然科学基金资助(项目编号050430604)

摘　　要：目的探讨人白细胞介素29（hIL-29）对Hela细胞TLR3受体表达的影响。方法重组的hIL-29加入Hela细胞中，以RT—PCR分析Toll样受体3（TLR3）、2’5’-寡聚腺苷酸合成酶（2＇5＇-OAS）、MXA蛋白的mRNA表达水平变化，western—blot分析TLR3受体蛋白质水平变化及信号蛋白MAPK（ERK1／2）激活变化。结果hIL-29显著上调TLR3受体的mRNA水平和蛋白质水平的表达，并且具有剂量依赖性。hIL-29刺激Hela细胞18h后，2’5’-OAS、MXA蛋白的mRNA表达水平显著升高（P〈0.01）。western—blot分析hIL-29刺激Hela细胞5～30min后，信号蛋白ERK1／2磷酸化水平显著升高（P〈0.01）。结论hIL-29可能通过激活ERK1／2上调（2＇5＇-OAS）、MXA和TLR3的表达。Purpose To explore the human Interleukin -29 （hIL-29） regulating the expression of TLR3 in Hela cell. Methods RT-PCR analyzes the effect of hIL-29 on the mRNA level of the TLR3, 2＇5＇-OAS,MXA in Hela cell treated with various concentrations of hIL-29 for 18 h. The protein level change of TLR3 was analyzed by western-blot, and the signal protein activated was detected by western-blot too. All experiments were performed in at least three independent assays. Blots and agarose electrophoresis were quantitated using BIOID software （SIM）. Data are summarized as x ± s. Statistical analysis of the results was performed by SPSS 10,0 software. Results The hIL-29 significantly up-regulates the mRNA and protein expression of TLR3 at a dose-dependent ways （ P 〈 0.01 ）. The hIL-29 also increased the expression of 2＇5＇-OAS and MXA in Hela cell treated with hHIL-29 for 18h （ P 〈 0.01 for comparison to the untreated control）. The phosphorylation of ERK1/2 kinases increased after the Hela cell was treated with hIL-29 for 5-30 min （P 〈 0. 01 ）. Conclusion The hIL-29 up-regulates the expression of 2＇5＇-OAS, MXA and TLR3 in Hela cell involved ERK1/2 activated.

关 键 词：人白介素(hIL)-29 TLR3 ERK1/2 

分 类 号：R392[医药卫生—免疫学]