TGF-β_1在肾病综合征患者血、尿及肾组织中表达的研究  

Research of expression of TGF-beta 1 in blood,urine and tissues of kidney of patient with nephrotic syndrome

作　　者：张亮[1] 华平[2] 郎学聪 何光莲 林会英 

机构地区：[1]浙江省舟山市人民医院肾内科,316004 [2]四川省宜宾市第二人民医院病理科,644000 [3]四川省宜宾市第二人民医院肾内科,644000

出　　处：《重庆医学》2007年第24期2522-2523,I0025,共3页Chongqing medicine

基　　金：四川省宜宾市重点科技计划项目(200403007)

摘　　要：目的探讨转化生长因子β_1(TGF-β_1)在肾病综合征患者血、尿及肾组织中的表达及其在发病中的作用。方法采用常规病理、免疫组织化学方法对肾组织进行染色,并经病理图像分析系统进行分析;血、尿TGF-β_1水平采用酶联免疫吸附法测定。结果肾病组患者的血、尿中TGF-β_1的含量明显高于正常对照组(P<0.001);TGF-β_1在正常对照组的肾小球无表达,而在肾病组患者肾小球及肾小管中有较密集的棕黄色颗粒,图像分析系统计算显示肾病组肾组织中的TGF-β_1表达较正常对照组显著增多,差异具有统计学意义(P<0.001)。结论肾病综合征患者血、尿及肾组织中TGF-β_1表达显著增加,可能在肾脏纤维化过程中发挥重要作用。Objective To explore the expression of transforming growth factor β1 （TGF-β1） in blood, urine and tissues of kidney of the patients with nephrotic syndrome and the role on its onset. Methods Histopathological and immunohistochemical methods and image analysis system were used to evaluate the expression and change of TGF-β1 in the tissues of kidney. The level of TGF-β1 of the blood and urine were detected by enzyme-linked immunosorbent assay. Results The expression of TGF-β1 in blood and urine was much higher in the patients with nephrotic syndrome than the normal control（P〈0. 001）. TGF-β1 did not express in the glomcrulus of normal control group,however,expressed more intensive yellow brown particles in the glomcrulus and nephric tubule of the patients with nephrotic syndrome,and the expression of TGF-β1 of the patients with nephrotic syndrome was significantly increased compared with the normal control group（P〈0. 001）. Conclusion The expression of TGF-β1 in blood, urine and tissues of kidney of the patients with nephrotic syndrome is significantly increased,and may play an important role in the process of renal fibrous degeneration.

关 键 词：转化生长因子Β1 肾病综合征 肾组织 

分 类 号：R692.3[医药卫生—泌尿科学]