白腐菌预处理对丙酸蒸解玉米芯制备纤维素的影响  

Influence of White-rot Fungi Pretreatment of Corncobs on Vapor-hydrolysis with Propionic Acid to Prepare Cellulose

作　　者：赵华[1] 丁涓[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《林产化学与工业》2007年第6期71-76,共6页Chemistry and Industry of Forest Products

摘　　要：探讨了白腐菌预处理对丙酸蒸解法制取纤维素的影响。在白腐菌对玉米芯进行预处理过程中,赖锰过氧化物酶(MnP)、木质素过氧化物酶(LiP)、漆酶、木聚糖酶和纤维素酶的酶活分别在第5、6、7、10和15 d达到最高值,分别为1.326、10.25、0.062 7、0.33和403 U/g。利用响应面分析法确定丙酸蒸解玉米芯最佳工艺条件为:料液比1∶10(g∶mL),蒸解时间70 min,丙酸质量浓度900 g/L,产物中纤维素的质量分数为91.09%。玉米芯经白腐菌预处理10 d后再用丙酸进行蒸解处理,产物中纤维素的质量分数和保留率分别为97.12%和94.70%,而半纤维素和木质素的质量分数仅为0.96%和0.92%。The influence of whlte-rot fungi pretreatment of corncob on vapor-hydrolysis with propionic acid to make cellulose was studied. The results showed that manganese peroxidase（MnP）, lignin peroxidase （LIP）, laccase, xylanase and ceIIulase treatments reached their biggest enzyme activities during the bio-treatment processing of corncob after 5, 6, 7, 15 and 10 d respectively, which were 1. 326, 10.25, 0. 062 7, 0.33 and 403 U/g, respectively. The optimized conditions of vapor-hydrolysis with propionic acid on corncob using response surface methodology were as follows： ratio of solid to liquid 1 ： 10 （g： mL） , 70 min, concentration of propionic acid 900 g/L, the content of cellulose reached 91.09 % of the product. After bio-treatment for 10 d, content and retention rate of cellulose were 97.12 % and 94.70 % , respectively, while contents of hemicellulose and lignin were 0.96 % and 0.92 % , respeetively.

关 键 词：玉米芯 白腐菌 丙酸 蒸解 纤维素 

分 类 号：TQ353.421[化学工程]