高渗盐水对大鼠肢体缺血再灌注致心肌损伤的保护作用  

The cardioprotective effects of hypertonic saline for rat myocardium injury following limbs ischemia reperfusion

作　　者：刘红[1] 海克蓉[2] 韩洪伟[1] 张焱[3] 马聚峰[1] 

机构地区：[1]宁夏医学院附属医院,宁夏银川750004 [2]宁夏自治区人民医院,宁夏银川750021 [3]宁夏医学院,宁夏银川750004

出　　处：《宁夏医学杂志》2007年第12期1059-1060,共2页Ningxia Medical Journal

摘　　要：目的观察大鼠肢体缺血再灌注(LIR)后心肌损伤的发生以及静脉输注7.5%高渗盐水(HS)的心肌保护作用。方法将24只SD大鼠随机分为3组:正常对照组(C组)、模型对照组(M组)、模型+高渗盐水处理组(H组),分别行心肌的超微机构观察;批量检测心肌超氧化物岐化酶(SOD)活性,丙二醛(MDA)含量。结果C组超微结构基本完好,M、H组均出现线粒体损害,H组较M组损害重,M组MDA明显高于其他两组(P<0.05),而SOD活性低于其他两组(P<0.05),H组与C组间比较有差异(P<0.05)。结论大鼠肢体缺血再灌注后发生了心肌损伤,静脉输注高渗盐水有一定的心肌保护作用。Objective To observe myocardium injury following limbs ischemia reperfusion （LIR） and the cardioprotective effects of hypertonic saline （HS） infusion. Methods 24 Spragne - Dawley rats were randomly divided into 3 groups： normal control group（ C group）, LIR model group （M group）, and LIR ＋ HS treatment group （H group）. Uhramicrostructure changes of myocardium tissue were observed. Superoxide dismutase （SOD） activities, malondialdehyde （MDA） contents were respectively measured. Results The ultramicrostructure in C group was normal. But it revealed different degree pathologic lesions in both M and H group, while the severity of myocardium damage was lessened in H group. Myocardium MDA content increased significantly in M group than those in C and H groups, meanwhile SOD activities decreased. And the changes of SOD activities between H group and C groups were significant, too. Conclusion Intravenous infusion of HS has some cardioprotective effects in myocardium injury occurred in rats after LIR.

关 键 词：大鼠 再灌注损伤 心肌损伤 高渗盐水 治疗作用 

分 类 号：R541[医药卫生—心血管疾病]