乌司他丁对呼吸机所致肺损伤大鼠体内炎性因子的影响  

Impact of ulinastatin on cytokine production in ventilation-induced lung injury in rat

作　　者：肖刚[1] 赵文静[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《徐州医学院学报》2007年第12期780-783,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省科技厅社会发展基金(BS2005019)

摘　　要：目的观察乌司他丁对呼吸机所致肺损伤大鼠体内炎性因子的影响。方法24只SD大鼠随机分为对照组、大潮气量通气组和乌司他丁处理组,每组8只。大潮气量通气组和乌司他丁处理组接受机械通气,通气参数均设为:潮气量30 m l/kg,呼吸频率40次/m in,吸呼比1∶3,吸入氧浓度21%。机械通气4 h后分别测定支气管肺泡灌洗液(BALF)中的蛋白含量、白细胞总数以及血清和BALF中肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6和IL-10的水平。结果大潮气量通气组大鼠BALF中蛋白含量、白细胞总数以及血清和BALF中TNF-α、IL-1β、IL-6水平均显著高于对照组和乌司他丁处理组,但IL-10水平则显著降低。乌司他丁处理组与对照组比较上述指标差异无显著性。结论乌司他丁可以抑制大潮气量机械通气大鼠体内促炎因子的产生,对呼吸机所致的生物伤有预防作用。Objective To investigate the impact of ulinastatin on the cytokine production in ventilation - induced lung injury in rats. Methods Twenty - four SD rats were randomly assigned to three groups （ n = 8 each ） ： sham group, large tidal volume ventilation group and ulinastatin pretreatment group. The set parameters of mechanical ventilation were VT = 30 ml/kg, RR = 40 breathes/min, I/E = 1：3 and FiO2 = 21%. After 4 h of ventilation, the concentration of protein and the total count of leukocyte in bronchoalveolar lavage fluid （BALF） were determined, as well as the levels of TNF-α, IL - 1/3, IL - 6 and IL - 10 in the serum and BALF. Results The concentration of protein and the total count of leukocyte in BALF were significantly increased in large tidal volume ventilation group. Compared to that in the sham group and ulinastatin group, the levels of TNF -α, IL - 113 and IL - 6 in serum and BALF were markedly elevated in the large tidal volume ventilation group, but the level of IL - 10 was reduced. No significant differences in the above indexes were found between the sham group and the ulinastatin pretreatment group. Conclusion Ulinastatin could attenuate the lung injury from large tidal volume ventilation through suppressing the production of cytokines.

关 键 词：机械通气 生物伤 细胞因子 乌司他丁 

分 类 号：R563[医药卫生—呼吸系统]